POLYATOMIC PRIMARY BEAM FOR SIMS

用于 SIMS 的多原子主光束

• 批准号: 3505405
• 负责人: Constance M John
• 金额: $ 5万
• 依托单位: CHARLES EVANS AND ASSOCIATES
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3505405
• 关键词: animal tissue; bioengineering /biomedical engineering; image enhancement; mass spectrometry; particle beam; polyion

DESCRIPTION (Adapted from the applicant's abstract): The Phase II NIH SBIR grant in ion imaging analysis of biological matrices awarded to Charles Evans and Associates, is producing very promising results. This research uses TOF-SIMS which stigmatically focuses secondary ions produced by bombardment with a Cs+ primary beam. This instrumentation can also produce ion images using a rastered microfocussed primary ion beam, a liquid metal ion gun which produces 69Ga+. SIMS analysis of various biomolecules in tissue-like model matrices using the monatomic primary ion beams is not producing sufficient secondary ions for high quality ion imaging of molecules at the concentrations typically encountered in tissue. In other laboratories increases in secondary ion yields of more than an order of magnitude have been obtained using polyatomic, rather than monatomic, primary ion beams. The first objective of this Phase I research is to adapt the TOF-SIMS mass spectrometer to a polyatomic primary ionbeam source and produce a broad beam of Au cluster ions. With this primary beam, ion imaging could be performed by stigmatically focussing the secondary ions. The second objective is to determine the detection limits and secondary ion yields of selected biomolecules in the tissue-like model matrices and, also in tissue samples, and compare themwith those obtained with monatomic primary ion beams.

描述（改编自申请人的摘要）：II阶段NIH SBIR Grant在授予的生物矩阵的离子成像分析中 查尔斯·埃文斯（Charles Evans and Associates）正在产生非常有希望的结果。 这 研究使用TOF-SIMS，将产生的二级离子焦化 通过用CS+主梁轰击。 该仪器也可以 使用栅格的微角色原代离子束产生离子图像，A 产生69GA+的液态金属离子枪。模拟人生分析各种 使用单原性原代的组织样模型矩阵中的生物分子 离子束没有产生足够的高质量辅助离子 分子的离子成像在通常遇到的浓度下 组织。 在其他实验室中，次要离子产量增加 使用多原子，已经获得的数量级不仅仅是一个数量级 而不是原始离子束。 第一个目标 第一阶段的研究是将TOF-SIMS质谱仪调整为 多原子初级离子源并产生宽光束 簇离子。 使用此主梁，可以执行离子成像 通过污名化将二级离子聚焦。 第二个目标是 确定所选的检测极限和二级离子产率 组织样模型矩阵中的生物分子，也在组织中 样品，并将它们与原子质原始离子获得的样品进行比较 梁。