T4N5 LIPOSOMES FOR PREVENTION OF SKIN CANCER

应用遗传公司开发了一种增加DNA修复的新方法 通过局部传递DNA修复酶在皮肤中。的具体目的 第一阶段的研究是衡量增强的 进行动物光致癌实验前的DNA修复 在第二阶段。噬菌体T4的UV-DNA特异性内切酶V是 经核酸内切酶处理导入紫外线照射的细胞 被包裹在脂质体中，也通过基因转染，导致 提高DNA修复能力。第一阶段将确定增强修复是否 在防止紫外线诱变方面安全有效。自发辐射和紫外线辐射 脂质体处理和转染人细胞的诱变频率 将会被测量。此外，脱除了嘧啶和6-4 照片产品和由此产生的修复补丁大小将以 这些细胞。

Applied Genetics Inc. has developed a new method for increasing DNA repair in skin by topical delivery of DNA repair enzymes. The specific aim of the Phase I research is to measure the extent and fidelity of the enhanced DNA repair before proceeding to photocarcinogenesis experiments in animals in Phase II. The UV-DNA specific endonuclease V from bacteriophage T4 was introduced into UV-irradiated cells by treatment with endonuclease encapsulated in liposomes and also by gene transfection, resulting in increased DNA repair. Phase I will determine if the enhanced repair is safe and effective in preventing UV mutagenesis. The spontaneous and UV- induced mutation frequency in liposome treated and transfected human cells will be measured. In addition the removal of both pyrimidine and 6-4 photoproducts and the resulting repair patch size will be measured in these cells.