ACOUSTIC VELOCITY GRADIENT FRACTIONATION

描述（根据申请人摘要改编）：总体目标 该方案的主要目的是设计、开发一种新的细胞分离技术， 这将提供更快速和改进细胞分离 包括外周血、骨髓。 在第一阶段， 申请组织（技术研究协会，公司，TRA）具有 建造并测试了一种装置， 使用专利的伪驻波超声波技术进行尺寸调整。 该技术不是在重力场中沉淀细胞， 声速梯度使细胞悬浮在柱子上 流体。 倒置柱消除了传统的问题， 与单位重力沉降有关的“流动极限”， 不需要繁琐的密度梯度形成。 设备简单 没有活动部件。 声速梯度分级提供了 一种独特的方法，它不仅可以区分细胞， 根据大小和形状，但通过内部结构的差异。 这项技术一旦完善， 包括外周血在内的各种细胞群的分级分离， 骨髓

DESCRIPTION (ADAPTED FROM APPLICANT'S ABSTRACT): The overall objective of this proposal is to design, develop a new cell separating technique that will provide a much more rapid and improved separation of cell populations including peripheral blood, bone marrow. In Phase I, the applicant organization (Technical Research Associates, Inc., TRA) has built and tested a device which rapidly separates bone marrow cells by size using patented pseudo-standing wave ultrasound technologies. Instead of sedimenting cells in a gravitation field, the technique used an acoustic velocity gradient to levitate the cells up through a column of fluid. The inverted column eliminated the traditional problem of "streaming limit" associated with unit gravity sedimentation and required no tedious density gradient formation. The apparatus is simple with no moving parts. Acoustic Velocity Gradient Fractionation provides a unique method which potentially discriminates between cells not only on the basis of size and shape but by differences in internal structure. This technology, when perfected, could provide a rapid and efficient fractionation of various cell populations including peripheral blood and bone marrow.