ULTRASOUND-ENHANCED INVERTED BONE MARROW FRACTIONATION

超声增强倒置骨髓分离

• 批准号: 3501928
• 负责人: OWEN D BRIMHALL
• 金额: $ 5万
• 依托单位: TECHNICAL RESEARCH ASSOCIATES, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-30 至 1991-03-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3501928
• 关键词: blood sedimentation test; bone marrow; cell sorting; density gradient ultracentrifugation; ultrasound; ultrasound biological effect

The overall objective of the proposed project is to design, develop and construct a bone marrow separation device that will offer significantly superior performance compared with current methods of velocity sedimentation. In Phase I Technical Research Associates (TRA) will apply patented pseudo-standing wave ultrasound technologies to separate bone marrow cells by size. This novel device will eliminate the traditional cell concentration problem referred to as the "streaming limit" associated with velocity sedimentation, using an acoustically inverted separation column. Using safe levels of ultrasound the forces generated by TRA's ultrasonic means are significantly higher than traditional "g" forces and will effect rapid cell separation. The use of ultrasonically generated velocity gradients will also eliminate the need for the tedious and difficult formation of density when integrated into commercial separation systems, will provide more efficient collection of specific bone marrow and other biological cells for clinical treatment. The device will also save time, decrease costs and provide unique performance for those interested in fractionation of bulk quantities of cells (or bone marrow components).

拟议项目的总体目标是设计、开发和 建造一个骨髓分离装置， 与当前速度方法相比，具有上级性能 沉淀 在第一阶段，技术研究助理（TRA）将申请 获得专利的伪驻波超声技术， 骨髓细胞的大小 这种新的设备将消除传统的 细胞浓度问题被称为“流限制”相关 速度沉降，使用声学反向分离 柱 使用安全水平的超声波， 超声波装置明显高于传统的“g”力， 将实现快速的细胞分离。 使用超声波产生的 速度梯度也将消除对繁琐和 当集成到商业分离中时，难以形成密度 系统，将提供更有效的收集特定的骨髓， 用于临床治疗的其他生物细胞。 该设备还将保存 节省时间，降低成本，并为那些对 大量细胞（或骨髓成分）的分级分离。