MEMBRANE AUTOTRANSFUSION SYSTEM (MATS)

术中血液回收(IBS)系统抽吸术中 手术伤口处，通过“细胞清洗”将其净化，并将其送回 同一个病人。我们开发了一种新的IBS设备，称为膜 使用中空纤维净化废血的自体输血(MAT) 用薄膜过滤器代替离心机。涉及的第一阶段计划 利用牛血进行的体外研究建立了这种手术 垫子的特点。两项动物研究也于#年完成。 I期显示回收的血细胞(RBC、WBC和 血小板)保留了其正常的功能，而垫片程序 没有明显的副作用。 第二阶段计划建议对十头小牛的垫子进行进一步评估。这个 动物实验的目的是模拟开放的受控出血 手术伤口部位，使垫片手术可应用于抢救 他流了很多血，然后把血重新注入动物体内。其他人的相互作用 诸如心肺搭桥和氧合器之类的系统在这里被取消 程序，使得只有垫子程序对动物的影响才能 要下定决心。每台机器将处理大约5升血液 做实验。棚子的血液样本和纯化的血液将被 接受血液学和细胞功能测试。动物的健康 术后监测一周。这项研究预计将 确定MAT程序的效率和安全特性。

Intraoperative blood salvage (IBS) system aspirates waste blood from surgical wound site, purifies it by "cell-washing", and returns it to the same patient. We developed a new IBS device called membrane autotransfusion (MAT) which purifies the waste blood using a hollow fiber membrane filter instead of a centrifuge. Phase-I program involved in-vitro studies using bovine blood which established the operating characteristics of the MAT. Two animal studies were also completed in Phase-I which showed that the recovered blood cells (RBC, WBC, and platelets) retained their normal functions, and that the MAT procedure had no observable side effects. Phase-II program proposes to further evaluate the MAT in ten calves. The animal experiment is designed to simulate controlled bleeding in an open surgical wound site so that the MAT procedure could be applied to salvage the shed blood and reinfuse it to the animal. Interaction of other systems such as heart-lung bypass and oxygenators are eliminated in this procedure so that effects of only the MAT procedure on the animal could be determined. About 5 liters of blood will be processed in each experiment. Blood samples of the shed and the purified blood will be subjected to hematological and cell function tests. Health of the animal will be monitored for one week post-operation. This study is expected to establish the efficiency and the safety features of the MAT procedure.