CONTINUOUS OXYGEN TENSION & PERFUSION MEASUREMENT SYSTEM

组织氧合和灌注及其相互关系是 最重要的生理参数和最难测量的 体内测量。 在第一阶段，我们验证了合并的可行性， 在微型混合热敏电阻/极谱传感器中， 增强型热扩散探针（ETDP）的能力和最近的 氧张力测量的进展。 第二阶段的开发工作 将完善和改进设计的原型探头/仪器系统 在第一阶段，以满足这一关键需求。 ETDP仪器将 扩展至包括组织氧张力测量至+0.3 mmHg， 加上温度（+0.003oC）和灌注（+10%）的测量， 具有适当的隔离以用于医疗应用。 一根铂丝 被封装在玻璃涂层的热敏电阻珠，凹进 电化学，镀金，作为一个流量不敏感的氧气 传感器. 将使用水性凝胶填充极谱仪的凹部。 阴极以提供对蛋白质降解的免疫力。 氧张力和 灌注将在连续或瞬时操作中测量。 模式，以提供准确性和灵活性。 的温度依赖性 的氧张力测量将被确定，并且测量 将通过软件进行真实的实时修正。 该综合系统将 允许研究灌注、温度和氧分压（以及 它们的相互关系）在相同的小组织体积中。 这种能力 是许多研究和临床研究的基础，包括 确定药理学操作的有效性， 不同的实验和临床状态的循环休克（a 每年造成超过25万美国人死亡的疾病）; 检查局部灌注和氧分压对 室性心律失常;以及监测各种形式的 冠状动脉闭塞的溶栓治疗。 预商用 我们将对这种综合混合动力系统进行评估， 技术评估和应用小组在整个第二阶段。

Tissue oxygenation and perfusion and their interrelationships are among both the most important physiologic parameters and the most difficult to measure in vivo. During Phase I, we verified the feasibility of combining, in a miniature hybrid thermistor/polarographic sensor, both the unique capability of the Enhanced Thermal Diffusion Probe (ETDP) and recent advances in oxygen tension measurements. This Phase II development effort will be refine and improve the prototype probe/instrument system designed in Phase I to meet this critical need. The ETDP instrumentation will be expanded to include the measurement of tissue oxygen tension to +0.3 mmHg, adding to the measurement of temperature (+0.003oC) and perfusion (+10%), with appropriate isolation for medical applications. A platinum wire will be encapsulated in the glass coating of a thermistor bead, recessed electrochemically, and gold plated to serve as a flow-insensitive oxygen sensor. Aqueous gels will be used to fill the recess of the polarographic cathode to provide immunity to protein degradation. Oxygen tension and perfusion will be measured in either continuous or transient operational modes to provide both accuracy and flexibility. The temperature dependence of the oxygen tension measurement will be determined, and the measurement will be corrected in real time via software. This integrated system will permit investigation of perfusion, temperature, and oxygen tension (and their interrelationships) in the same small tissue volume. This capability is fundamental to many research and clinical studies, which include determination of the effectiveness of pharmacologic manipulations in different experimental and clinical states of circulatory shock (a condition responsible for over 250,000 Americans deaths yearly); examination of the effect of local perfusion and oxygen tension on ventricular arrhythmias; and monitoring of the various forms of thrombolytic therapy for coronary artery occlusion. Pre-commercial evaluation of this integrated hybrid system will be carried out by our Technology Assessment and Applications Group throughout Phase II.