Studies on biosensor device technology based on immobilization of intact liposome on Si-integrated sensor structure

基于硅集成传感器结构固定完整脂质体的生物传感器器件技术研究

• 批准号: 19360162
• 负责人: NODA Minoru
• 金额: $ 12.15万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19360162/
• 关键词: バイオセンサ; マイクロナノデバイス; 脂質; バイオ関連機器; バイオテクノロジー; リポソーム; 固定化; 親水性; 表面酸化; SAM (self-assembled mono-layer); 熱化学センサ; ボロメータ

We have studied a series of biosensor device technologies based on immobilization of intact liposome on Si-integrated sensor structure. As a result, the immobilized liposome keeps its intact behavior more than 24hrs, meaning practically long time range for usual sensing. Based on the result, three type of liposome biosensor of microbolometer biothermochemical sensor, leakage current sensor and cantilever strain sensor are designed, fabricated and evaluated for detection of target proteins. Finally these sensors are confirmed to show their effective sensing performance.

我们研究了一系列基于完整脂质体固定在硅集成传感器结构上的生物传感器器件技术。结果，固定化脂质体保持其完整的行为超过24小时，这意味着对于通常的传感来说，几乎是很长的时间范围。在此基础上，设计、制作和评价了微测辐射热计生物热化学传感器、泄漏电流传感器和悬臂梁应变传感器三种类型的脂质体生物传感器对目标蛋白质的检测。最后对这些传感器进行了验证，证明了它们的有效性。

项目成果

Bio-Thermochemical Sensor with Liposome Immobilized Intact for Protein Detection Using Their Interaction and Membrane Dynamics

完整固定脂质体的生物热化学传感器，利用其相互作用和膜动力学进行蛋白质检测

• 发表时间: 2008
• 期刊: Proc. IEEE Sensors 2008 1
• 作者: Minoru Noda;Takeshi Asai;Kaoru Yamashita;Toshinori Shimanouchi;Masanori Okuyama;Ryouichi Kuboi
• 通讯作者: Ryouichi Kuboi

A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein"

通过检测电解质捕获脂质体与蛋白质之间相互作用的漏电流来构建蛋白质微传感器"

• 发表时间: 2009
• 作者: M. Noda;T. Asai;T. Shimanouchi;K. Yamashita;H. Umakoshi;M. Okuyama;R. Kuboi
• 通讯作者: R. Kuboi

A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein

通过检测电解质捕获脂质体与蛋白质之间相互作用的漏电流来构建蛋白质微传感器

• 发表时间: 2009
• 作者: M.Noda;T.Asai;T.Shimanouchi;K.Yamashita;H.Umakoshi;M.Okuyama;R.Kuboi
• 通讯作者: R.Kuboi

電解質封入リポソームータンパク質問相互作用検出漏れ電流マイクロセンサ

电解质封装脂质体-蛋白质相互作用检测漏电流微传感器

• 发表时间: 2009
• 作者: 有延康;浅井健;山下馨;野田実;島内寿徳;馬越大;奥山雅則;久保井亮一
• 通讯作者: 久保井亮一

Biosensing of interaction between liposome and protein evaluated with microwave dielectric dispersion analysis

用微波介电色散分析评估脂质体和蛋白质之间相互作用的生物传感

• 发表时间: 2008
• 作者: Y. Masuda;K. Morii;T. Shimanouchi;K. Yamashita;M. Okuyama;R. Kuboi;M. Noda
• 通讯作者: M. Noda