MINORITY HIGH SCHOOL STUDENT RESEARH APPRENTICE PROGRAM

德克萨斯理工大学健康科学中心有一个数字， 目前从事研究的教师， 复杂混合物的分离、检测和分析 proteins. 其中一些调查人员正在使用两个- 双向凝胶电泳（2-D PAGE），然后用银- 染色、放射自显影或荧光照相来完成这一点 分离和检测。 这份报告中列出的主要调查人员 有人建议使用2-D PAGE来检测蛋白质 这可能是重要的调节类固醇， 控制与植入有关的事件， 在调节生长，发育和新陈代谢的 骨骼肌，蛋白质，可能是合成的结果 雌激素对子宫的作用，外膜蛋白， 由E.杆菌和 最后，可能参与细胞识别的蛋白质 在蝌蚪尾退化时被移除。 的实例 在几个实验中产生的二维凝胶的放射自显影图和荧光图 这些研究的结果都出现在本提案的案文中。 蛋白质的分离和检测方法 目前正在使用上述方法，但是， 除了目视检查之外，尚不可能对2-D凝胶进行分析， 缺乏必要的设备。 因此，这一提议是 申请资金购买一台电脑辅助两台 三维凝胶扫描设备，这是必不可少的 成功分析本文所述的蛋白质模式。 这些扫描系统可商购获得，并且能够 对蛋白质进行定量和定性分析 通过2-D PAGE分离。 除了二维凝胶扫描， 我们预计，这种仪器也将用于 1-D凝胶和斑点印迹分析以及 显微照片上的细胞、亚细胞器和颗粒 这些数据正在被一个不那么复杂和缓慢的 TTUHSC目前使用的“Bioquant”成像系统。

The Texas Tech University Health Sciences Center has a number of faculty who are currently engaged in research which requires the separation, detection and analysis of complex mixtures of proteins. Several of these investigators are using two- dimensional gel electrophoresis (2-D PAGE) followed by silver- staining, autoradiography or fluorography to accomplish this separation and detection. The major investigators listed in this proposal are using 2-D PAGE in an attempt to detect proteins which may by important in the regulation of steroidogenesis, the control of events involved in implantation, those which may serve in regulating the growth, development and metabolisms of skeletal muscle, proteins which may be synthesized as a result of estrogen action on the uterus, outer membrane proteins which are synthesized and secreted by various mutants of E. coli, and lastly, proteins which may be involved in the recognition of cells to be removed during tadpole tail regression. Examples of autoradiograms and fluorograms of 2-D gels generated in several of these studies appear in the text of this proposal. The methodology for the separation and detection of proteins mentioned above is currently being utilized, however, the analysis of 2-D gels other than visual is not yet possible due to a lack of the necessary equipment. Therefore, this proposal is requesting funds to purchase a computer assisted two- dimensional gel scanning device which is essential to the successful analysis of the protein patterns described herein. These scanning systems are available commercially and are able to perform both quantitative and qualitative analyses of proteins separated by 2-D PAGE. In addition to the scanning of 2-D gels, we anticipate that this instrumentation will also be used for the analysis of 1-D gels and dot-blots as well as for image analysis of cells, subcellular organelles and particles on photomicrographs which are being analysed by a less sophisticated and much slower "Bioquant" imaging system currently in use at TTUHSC.