MULTIPLE ZONE PULSED FIELD DNA SEQUENCING

多区脉冲场 DNA 测序

• 批准号: 2208971
• 负责人: Eric H. LAI
• 金额: $ 10万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-03 至 1995-05-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2208971
• 关键词: DNA; pulsed field gel electrophoresis

This is a Shannon Award providing partial support for research projects that fall short of the assigned institute's funding range but are in the margin of excellence. The Shannon award is intended to provide support to test the feasibility of the approach; develop further tests and refine research techniques; perform secondary analysis of available data sets; or conduct discrete projects that can demonstrate the PI's research capabilities or lend additional weight to an already meritorious application. Further scientific data for the CRISP System are unavailable at this time.

这是一个为研究项目提供部分支持的香农奖 低于指定研究所的资助范围，但在 卓越边际。香农奖旨在提供支持 测试该方法的可行性；开发进一步的测试和改进 研究技术；对现有数据集进行二次分析； 或进行独立的项目，以证明PI的研究 能力或为已经功成名就的 申请。CRISP系统的进一步科学数据是 目前不可用。

项目成果

Use of secondary pulsed field gel electrophoresis in separation of large DNA.

使用二次脉冲场凝胶电泳分离大 DNA。

• DOI: 10.1006/abio.1995.1009
• 发表时间: 1995
• 期刊: Analytical biochemistry.
• 作者: Lai,E;Birren,BW
• 通讯作者: Birren,BW

Pulsed field separation of large supercoiled and open-circular DNAs and its application to bacterial artificial chromosome cloning.

大型超螺旋和开环 DNA 的脉冲场分离及其在细菌人工染色体克隆中的应用。

• DOI: 10.1002/elps.1150160102
• 发表时间: 1995
• 期刊: Electrophoresis.
• 作者: Wang,M;Lai,E
• 通讯作者: Lai,E

Rapid pulsed field separation of DNA molecules up to 250 kb.

快速脉冲场分离高达 250 kb 的 DNA 分子。

• DOI: 10.1093/nar/22.24.5366
• 发表时间: 1994
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Birren,B;Lai,E
• 通讯作者: Lai,E