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Typing of Staphylococcus aureus and Staphylococcus intermedius by pulsed -field gel electrophoresis, ribotyping, and random amplified polymorphic DNA analysis

通过脉冲场凝胶电泳、核糖分型和随机扩增多态性 DNA 分析对金黄色葡萄球菌和中间葡萄球菌进行分型

基本信息

批准号：
08660381
负责人：
SHIMIZU Akira
金额：
$ 1.47万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

The purpose of this study was to evaluate the discriminatory power of pulsed-field gel electrophoresis (PFGE), ribotyping, random amplified polymorphic DNA (RAPD), and phage typing for differentiating of strains of S.aureus, S.intermedius and S.hyicus isolated from animals.1. Specific PFGE patterns were observed according to the animal species (human, cattle, chicken, pig and horse), suggesting that ecovars based on the genetic background may be present in S.aureus. 2. There were some differences in PFGE patterns among countries (S.aureus from chickens in England, Belgium, Bulgaria, Argentina and Japan). The PFGE appears to be a useful method for subtyping strains of avian phage groups or poultry specific ecovar. 3. PFGE was used to determine genetic relationships among 15 MRSA isolates from mares with metritis. All the 15 isolates showed the same PFGE pattern, suggesting that they have originated from a single clone. 4. The outbreak of S.aureus infection occurring on the same poultry farm involved S.aureus with different PFGE patterns. 5. Strains of S.intermedius from mink, foxes, pigeons and dogs were typed by PFGE,ribotyping, RAPD and phage typing. Specific PFGE patterns were observed according to the animal species, suggesting that ecovars based on the genetic background may be present in S.intermedius. Also, separation of these strains was possible when using ribotyping and RAPD.The PFGE appears to be a useful method for subtyping strains of ribotypes or phage types. The PFGE and ribotyping could be used to distinguish between S.intermedius and S.aureus. 6. Strains of S.hyicus isolated from horses showed some deviation from pig or chicken strains with respect to their PFGE patterns. We conclude that PFGE analysis is a useful tool not only for species or strain identification but also for epidemiologic or ecologic studies of S.aureus, S.intermedius and S.hyicus.

本研究的目的是评价脉冲场凝胶电泳(PFGE)、核糖体分型、随机扩增多态性DNA(RAPD)和噬菌体分型在区分动物分离的金黄色葡萄球菌、中间葡萄球菌和海链霉菌中的鉴别能力。根据动物种类(人、牛、鸡、猪和马)观察到特定的PFGE图谱，表明金黄色葡萄球菌可能存在基于遗传背景的生态变种。2.不同国家(英国、比利时、保加利亚、阿根廷和日本的鸡源金黄色葡萄球菌)的PFGE图谱存在一些差异。对于禽噬菌体群或禽类特异性生态变种的亚型，PFGE似乎是一种有用的方法。3.应用聚合酶链式反应-凝胶电泳法(PFGE)对15株子宫炎马的MRSA分离株进行了亲缘关系分析。所有15个分离物都表现出相同的PFGE图谱，表明它们来自单个克隆。4.同一养鸡场发生的金黄色葡萄球菌感染涉及不同PFGE图谱的金黄色葡萄球菌。5.对水貂、狐狸、鸽子和狗的中间链球菌进行了PFGE、核糖体分型、随机扩增多态性DNA和噬菌体分型。根据动物种类观察到特定的PFGE图谱，表明中间链霉菌可能存在基于遗传背景的生态变种。此外，当使用核糖体分型和RAPD时，这些菌株的分离是可能的。PFGE似乎是一种有用的方法，用于核糖型或噬菌体类型的菌株的亚型鉴定。PFGE和核糖体分型可用于区分中间葡萄球菌和金黄色葡萄球菌。6.马分离的猪链球菌与猪、鸡分离株的PFGE图谱有一定的差异。我们的结论是，PFGE分析不仅是鉴定物种或菌株的有用工具，而且也是金黄色葡萄球菌、中间葡萄球菌和猪链球菌的流行病学或生态学研究的有用工具。