MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

少数民族高中生研究学徒计划

• 批准号: 3512990
• 负责人: NICHOLAS SPERELAKIS
• 金额: $ 0.6万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3512990
• 关键词: minority institution research support; secondary schools

This application request funding to purchase the following instruments: 1) A DNA synthesizer with three column synthesis capability and full programability (Applied Biosystems model 380B-0s). 2) A peptide systhesizer with three reaction vessels and a single resin sampler (Applied Biosystems model 430A). 3) High pressure liquid chromatography system (Beckman model 344HPLC), variable wave-length detector (model 165), and integrator recorder (model 427). These instruments will be installed in the core molecular and cell biology laboratories which were constructed recently with NHLBI funding (ICO 6 HL 35669) and are going to serve the needs of the investigators of the Cardiovascular Institute, the Biophysics Institute, and the Pulmonary and Arthritis Center. The growing utilization of the techniques of molecular biology in biomedical research necessitated the recruitment of new faculty with expertise in molecualr biology and the development of post doctoral training programs in several clinical departments. There is a daily need to utilize the nucleotide synthesizer for studies involving the structure, function and the regulation of expression of important human genes (apolipoproteins, contractile proteins, acute phase proteins, and fatty acid binding proteins). Other proteins and genes are currently being characterized. Among these are alpha, adrenergic receptor, lipoprotein lipase, glutamate dehydrogenase, sodium potassium ATPase. Synthetic peptides will also be used extensively as inhibitors of proteolytic enzymes, renin converting enzymes, kallikrein and kinin, vassopressin analogs, etc. The HPLC units will be used for purification and analysis of the synthetic oligonucleotides and peptides. There is an institutional commitment for personnel and materials to operate the instruments for the first two years. Dr. James Burton, an expert in solid phase peptide synthesis, and Drs. Zannis, Ruiz-Opazo, and Roghani will supervise the initial installation and operation of these instruments. After the initial support from Boston University, most of the financial support for the operation of the instruments will be obtained from existing or new research grants. The costs will be prorated to take into consideration the extent of utilization of each scientist and research program. A similar mechanism for dividing costs between users is already in place for other core instruments at Boston University.

本申请要求拨款购买以下物品 仪器： 1)一种具有三柱合成能力的DNA合成仪， 完全可编程性（Applied Biosystems型号380 B-0 s）。 2)具有三个反应容器和单个反应容器的肽合成器 树脂取样器（Applied Biosystems型号430 A）。 3)高压液相色谱系统（Beckman型 344 HPLC），可变波长检测器（型号165），和 积分记录仪（型号427）。 这些仪器将安装在核心分子和细胞中 最近与NHLBI一起建造的生物实验室 资金（ICO 6 HL 35669），并将服务于 心血管研究所的研究人员， 研究所和肺关节炎中心。 日益增长的 分子生物学技术在生物医学中的应用 研究需要招聘新的教师， 分子生物学专业知识和岗位发展 在几个临床部门的博士培训计划。 每天都需要利用核苷酸合成仪来 研究涉及的结构，功能和调节 表达重要的人类基因（载脂蛋白、收缩性 蛋白、急性期蛋白和脂肪酸结合蛋白）。 其他蛋白质和基因目前正在表征中。 其中包括α，肾上腺素能受体，脂蛋白脂酶， 谷氨酸脱氢酶、钠钾ATP酶。 合成 肽也将广泛用作蛋白水解的抑制剂 酶，肾素转化酶，激肽释放酶和激肽， 加压素类似物等。HPLC装置将用于 合成的寡核苷酸的纯化和分析， 缩氨酸 有一个机构承诺， 在头两年使用仪器的材料。 博士 詹姆斯伯顿，固相肽合成专家，和博士。 赞尼斯，鲁伊斯-奥帕佐和罗加尼将监督最初的 这些仪器的安装和操作。 在最初的 波士顿大学的支持，大部分的财政支持， 仪器的操作将从现有的或 新的研究赠款。 费用将按比例计算， 考虑每位科学家的利用程度， 研究计划。 类似的成本分摊机制 其他核心文书的用户之间的联系已经到位， 波士顿大学。