NEONATAL LEAD EXPOSURE EFFECTS ON THE ADRENAL CORTEX FUNCTION

铅水平升高对脑组织功能的破坏性影响 发育不良的下丘脑-垂体-肾上腺轴(HPAA) 检查过了。这项研究调查的目的是检查 铅暴露对HPAA功能个体发育的影响在这 调查显示，Spraogue-Dawley大鼠出生后将接受铅注射 第1天(PND)至第21天。新生儿铅接触者血铅水平的研究 将对动物及其控制进行监测。所有的动物都会 在PND 21、35和120评估和评估肾上腺功能 使用不同的实验范式。在一个实验中，我们将 检查铅暴露对下丘脑功能的影响， 脑下垂体和肾上腺。在另一个实验中，我们将评估 用基础状态评价染铅动物的肾上腺皮质功能 皮质酮、促肾上腺皮质激素、糖皮质激素受体、下丘脑 生物胺、血糖水平和肝糖原。我们还将 检查急性和慢性应激对荷尔蒙水平的影响 这些动物。在最后的实验中，我们将评估有毒物质 铅对肾上腺皮质成熟的影响。其结果是 这项研究应该有助于理解新生儿如何暴露于 铅可以改变肾上腺皮质功能。

The disruptive effects of elevated lead levels on the functioning of developing hypothalamic-pituitary-adrenal axis (HPAA) has not been well examined. The purpose of this research investigation is to examine the effect of lead exposure on the ontogeny of HPAA function. In this investigation, Sprague-Dawley rats will be administered lead at postnatal day (PND) 1 until PND 21. Blood levels of lead in neonatally exposed animals and their control will be monitored. All animals will be assessed and evaluated at PND 21, 35 and 120 for the adrenal function using different experimental paradigms. In one experiment we will examine the effect of lead exposure on the function of the hypothalamus, pituitary and the adrenal gland. In another experiment we will evaluate the adrenocortical function of lead treated animals by evaluating basal levels of corticosterone, ACTH, glucocorticoid receptors, hypothalamic biogenic amines, plasma glucose levels and liver glycogen. We will also examine the effect of acute and chronic stress on the hormonal profile of these animals. In a final experiment we will evaluate the toxic effect of lead on the maturation of the adrenal cortex. The outcome of this research should help in the understanding of how neonatal exposure to lead can modify the adrenal cortical function.