NEONATAL LEAD EXPOSURE EFFECTS ON THE ADRENAL CORTEX FUNCTION

新生儿铅暴露对肾上腺皮质功能的影响

• 批准号: 3777891
• 负责人: KARAM F A SOLIMAN
• 金额: --
• 依托单位: FLORIDA AGRICULTURAL AND MECHANICAL UNIV
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3777891
• 关键词: adrenal glands; adrenocorticotropic hormone; atomic absorption spectrometry; blood glucose; corticosterone; endocrine disorder; glucocorticoids; glycogen; high performance liquid chromatography; hormone receptor; hypothalamic pituitary axis; iodine; laboratory rat; lead poisoning; neurotransmitters; newborn animals; pharmacokinetics; radionuclides; stress

The disruptive effects of elevated lead levels on the functioning of developing hypothalamic-pituitary-adrenal axis (HPAA) has not been well examined. The purpose of this research investigation is to examine the effect of lead exposure on the ontogeny of HPAA function. In this investigation, Sprague-Dawley rats will be administered lead at postnatal day (PND) 1 until PND 21. Blood levels of lead in neonatally exposed animals and their control will be monitored. All animals will be assessed and evaluated at PND 21, 35 and 120 for the adrenal function using different experimental paradigms. In one experiment we will examine the effect of lead exposure on the function of the hypothalamus, pituitary and the adrenal gland. In another experiment we will evaluate the adrenocortical function of lead treated animals by evaluating basal levels of corticosterone, ACTH, glucocorticoid receptors, hypothalamic biogenic amines, plasma glucose levels and liver glycogen. We will also examine the effect of acute and chronic stress on the hormonal profile of these animals. In a final experiment we will evaluate the toxic effect of lead on the maturation of the adrenal cortex. The outcome of this research should help in the understanding of how neonatal exposure to lead can modify the adrenal cortical function.

铅水平升高对脑功能的破坏性影响 下丘脑-垂体-肾上腺轴（HPAA）的发育一直不好， 考察 本研究调查的目的是检查 铅暴露对HPAA功能个体发育的影响。 在这 研究中，Sprague-Dawley大鼠将在出生后给予铅 第1天（PND）至PND 21。 经尿道接触者血铅水平 将监测动物及其对照。 所有动物 在PND 21、35和120时评估和评价肾上腺功能 使用不同的实验范例。 在一个实验中，我们将 检查铅暴露对下丘脑功能的影响， 垂体和肾上腺。 在另一个实验中，我们将评估 铅处理动物肾上腺皮质功能的基础评价 皮质酮、ACTH、糖皮质激素受体、下丘脑 生物胺、血浆葡萄糖水平和肝糖原。 我们还将 研究急性和慢性压力对荷尔蒙的影响 这些动物。 在最后的实验中，我们将评估 铅对肾上腺皮质成熟的影响。 的结果 这项研究应该有助于了解新生儿如何接触 铅会改变肾上腺皮质功能