RADIOANALYTIC IMAGING SYSTEM

这种共享工具赠款的目的是获得资金， 购买AMBIS图像采集和分析系统， 分子生物学系、细胞生物学系、 斯克里普斯诊所研究所的免疫学博士。研究所 现在有一些研究人员使用实验方法， 其涉及35 S-、14 C-、32 P-标记蛋白质的定量分析 以及使用一维和二维凝胶分离的核酸 电泳使用放射自显影的标准方法， 密度测定法缺乏灵敏度、线性和动态曝光范围， 生成准确的定量结果，无需仔细校准 或产生可变周期的多次曝光， 在膜的线性区域中获得可测量结果的时间。 所有 现在，拟议的研究中有10%需要使用AMBIS成像系统 其（1）适用于35 S（14 C）和32 P，（2）具有动态 5个数量级的范围，无需多个 曝光，（3）是大约20倍以上的敏感，然后标准 放射自显影方法，大大缩短了数据采集的时间 采集，和（4）是高度定量的，提供直接的CPM 在要求间隔小于0.4 mm的条带中进行测定 一维或二维凝胶上的Y轴。 购买放射性分析仪器 成像系统将消除我们目前对经典的依赖 放射自显影术和密度测定术及其相关问题， 由于胶片的不敏感性和在生产中的困难， 准确定量。

The purpose of this shared instrumentation grant is to obtain funds to purchase an AMBIS Image Acquisition and Analysis System to be used by investigators in the Departments of Molecular Biology, Cellular Biology and Immunology at the Research Institute of Scripps Clinic. The Institute now contains a number of investigators using experimental approaches which involve quantitative analysis of 35S-, 14C-, 32P-labelled proteins and nucleic acids separated using one- and two-dimensional gel electrophoresis. Standard approaches using autoradiography and densitometry lack sensitivity, linearity and a dynamic exposure range to generate accurate quantitative results without either careful calibration of the film, or generation of multiple exposures for variable periods of time to obtain measurable results in the linear region of the film. All of the proposed studies now require the use of a AMBIS Imaging system which (1) is applicable to both 35S (14C) and 32P, (2) has a dynamic range of 5 orders of magnitude, eliminating the need for multiple exposures, (3) is approximately 20-fold more sensitive then standard autoradiographic approaches, greatly reducing the time-period for data acquisition, and (4) is highly quantitative, providing direct cpm determination in bands which require separation of less than 0.4 mm in Y-axis on one- or 2-dimensional gels. Acquisition of a Radioanalytic Imaging System will eliminate our current dependence on classical autoradiography and densitometry with its associated problems in reduced productivity due to insensitivity of the film and difficulties in accurate quantitation.