RADIOANALYTIC IMAGING SYSTEM

放射分析成像系统

• 批准号: 3521783
• 负责人: William Edward Balch
• 金额: $ 12.4万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521783
• 关键词: DVD /CD ROM; biomedical equipment purchase; biomedical equipment resource; carbon; computer data analysis; computer human interaction; computer program /software; computer system hardware; image enhancement; phosphorus; radiation detector; radiography; radionuclides; sulfur

The purpose of this shared instrumentation grant is to obtain funds to purchase an AMBIS Image Acquisition and Analysis System to be used by investigators in the Departments of Molecular Biology, Cellular Biology and Immunology at the Research Institute of Scripps Clinic. The Institute now contains a number of investigators using experimental approaches which involve quantitative analysis of 35S-, 14C-, 32P-labelled proteins and nucleic acids separated using one- and two-dimensional gel electrophoresis. Standard approaches using autoradiography and densitometry lack sensitivity, linearity and a dynamic exposure range to generate accurate quantitative results without either careful calibration of the film, or generation of multiple exposures for variable periods of time to obtain measurable results in the linear region of the film. All of the proposed studies now require the use of a AMBIS Imaging system which (1) is applicable to both 35S (14C) and 32P, (2) has a dynamic range of 5 orders of magnitude, eliminating the need for multiple exposures, (3) is approximately 20-fold more sensitive then standard autoradiographic approaches, greatly reducing the time-period for data acquisition, and (4) is highly quantitative, providing direct cpm determination in bands which require separation of less than 0.4 mm in Y-axis on one- or 2-dimensional gels. Acquisition of a Radioanalytic Imaging System will eliminate our current dependence on classical autoradiography and densitometry with its associated problems in reduced productivity due to insensitivity of the film and difficulties in accurate quantitation.

该共享仪器赠款的目的是获得资金 购买AMBIS图像采集和分析系统要使用 分子生物学，细胞生物学系的研究者 Scripps诊所研究所的免疫学。研究所 现在使用实验方法包含许多研究者 涉及35s-，14c-，32p标记蛋白的定量分析 和使用一维凝胶分离的核酸 电泳。使用放射自显影和 光密度学缺乏灵敏度，线性和动态暴露范围 产生准确的定量结果，而无需仔细校准 胶片的生成或产生多个暴露于可变周期的 是时候在膜的线性区域中获得可测量的结果。 全部 现在提出的研究需要使用AMBIS成像系统 （1）适用于35s（14c）和32p，（2）具有动态 5个数量级的范围，消除了对多重的需求 暴露，（3）比标准更敏感大约20倍 放射自显影方法，大大降低了数据的时间段 获取，（4）具有高度定量，提供直接的CPM 在需要分离小于0.4 mm的频段中的测定 Y轴上或二维凝胶上。 获取放射分析 成像系统将消除我们当前对经典的依赖 放射自显影和光密度法及其相关问题减少 由于电影的不敏感而产生的生产力和困难 准确的定量。