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LASER SCANNING CONFOCAL MICROSCOPE AND WORKSTATION

激光扫描共焦显微镜和工作站

基本信息

批准号：
3521813
负责人：
Robert P Thompson
金额：
$ 18.5万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-08-15 至 1994-08-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3521813
关键词：
DVD /CD ROM biomedical equipment purchase biomedical equipment resource computer graphics /printing computer program /software computer system hardware confocal scanning microscopy lasers

项目摘要

A Biorad MRC100 laser scanning confocal microscope with Silicon Graphics IRIS Indigo workstation and selected peripherals are requested by a group of biomedical researchers at the Medical University of South Carolina. This instrument would bring new and powerful light microscopy and computer imaging technology directly to bear upon 10 active or pending NIH projects; no other such facilities exist within the Charleston scientific community, comprising some 120 NIH projects. Immediate applications of this instrumentation include high resolution colocalization of intracellular cytoskeletal or signalling molecules in several experimental cell culture or organ systems and delineation of the spatial distribution of extracellular matrix, adhesion molecules and other differentiation markers during development or in diseases tissues. Direct experience and pilot studies suggest novel applications in counting discrete immunolocalized features such as gap junctions or parasitic antigens over large volumes of tissue and three-dimensional representation of myocardial fiber geometry in dense tissue preparations. Available expertise in confocal microscopy and in conventional light microscopy and image processing among the major users assure full utilization of both the laser scanning confocal microscope and the imaging workstation. Reliable operation of the proposed facility and access to an active community of researchers in the future are assured by past and present collaborations among major users and a specific organizational plan including technical and central administrative support.

带 Silicon Graphic 的 Biorad MRC100 激光扫描共焦显微镜团体请求 IRIS Indigo 工作站和选定的外围设备南卡罗来纳医科大学的生物医学研究人员。该仪器将带来新的、强大的光学显微镜和计算机成像技术直接影响10个活跃或待决的事件美国国立卫生研究院项目；查尔斯顿酒店内不存在其他此类设施科学界，由大约 120 个 NIH 项目组成。即时该仪器的应用包括高分辨率细胞内细胞骨架或信号分子的共定位几个实验细胞培养物或器官系统和描述细胞外基质、粘附分子的空间分布发育期间或疾病组织中的其他分化标记。直接经验和试点研究表明了新的应用计算离散的免疫定位特征，例如间隙连接或大体积组织和三维的寄生抗原致密组织制剂中心肌纤维几何形状的表示。共焦显微镜和传统光方面的可用专业知识主要用户之间的显微镜和图像处理确保充分利用激光扫描共焦显微镜和成像工作站。拟议设施的可靠运行和确保将来能够接触到活跃的研究人员社区通过主要用户和特定用户之间过去和现在的合作组织计划，包括技术和中央行政支持。