ANALYTICAL ULTRACENTRIFUGE

已要求提供资金，以购买一个新开发的、最先进的 分析型超离心层析仪（Beckman XL-A）。 该工具将 替换现有的分析超滤仪（Beckman Model E）， 它已经有30多年的历史了， 保持。 研究小组由六名教师组成， 德克萨斯大学生物化学系 圣安东尼奥中心。 六名教师中的每一位都指导PHS资助的 涉及大分子结构研究的研究计划 和功能，每个都利用分析超离心作为一个 其研究活动的组成部分。 所要求的分析 超级计算机将提供一种稳定、可靠的手段来继续这些 实验，其中包括调查：1）染色质结构和 稳定性，2）细胞色素氧化酶单体-二聚体平衡，3）机制 氧化和折叠的线粒体酶rhodanese，4） 噬菌体的结构和构象，5）组装机制， 多酶复合物丙酮酸脱氢酶和6）相互作用 黄素蛋白和血红素蛋白在微粒体电子 细胞色素P450介导的转运系统。 除了更换 过时的技术，现代分析超级计算机的收购 将允许这六名研究人员进行各种生物分析， 研究计划，通过研究出版物， 分析超浓缩作为 现代研究工具。 这反过来将有助于重新教育 科学界对一种强大的技术的看法， 从今天的技术手段中消失了。

Funds have been requested to purchase a newly developed, state-of-the-art analytical ultracentrifuge (the Beckman XL-A). This instrument will replace an existing analytical ultracentrifuge (the Beckman Model E), which is over 30 years old, and has become virtually impossible to maintain. The investigator group consists of six faculty in the Department of Biochemistry at the University of Texas Health Science Center at San Antonio. Each of the six faculty directs PHS-funded research program(s) involving investigations of macromolecular structure and function, and each utilizes analytical ultracentrifugation as a component of their research activities. The requested analytical ultracentrifuge will provide a stable, dependable means to continue these experiments, which include investigations of: 1) chromatin structure and stability, 2) cytochrome oxidase monomer-dimer equilibrium, 3) mechanisms of oxidation and folding of the mitochondrial enzyme rhodanese, 4) bacteriophage structure and conformation, 5) mechanisms of assembly of the multienzyme complex pyruvate dehydrogenase and 6) the interactions between flavoproteins and heme proteins in the microsomal electron transport system mediated by cytochromes P450. In addition to replacing outdated technology, acquisition of a modern analytical ultracentrifuge will allow this group of six investigators with diverse bioanalytical research programs to demonstrate, through research publications, the usefulness and importance of analytical ultracentrifugation as a contemporary research tool. This in turn will help re-educate the scientific community about a powerful technique which has essentially disappeared from today's arsenal of technical approaches.