CHROMATIN STRUCTURE AND STABILITY IN THE SOLUTION STATE

描述：真核生物DNA组织的第一个层次是 在核小体中，它由大约200 bp的DNA包裹 围绕着组蛋白八聚体 核小体被认为是 染色体结构的基本元素。 加入其它 蛋白质进入寡核小体阵列产生特定的染色质 具有特定核功能的结构，如转录 活动 拟议研究的总体目标是确定和 表征核小体阵列的结构和功能状态 并了解组蛋白、连接组蛋白和 转录因子在指导和维持更高的秩序 核小体阵列折叠和自我折叠所带来的染色质结构， 协会 提出了三项具体任务， 假设核小体的内在构象动力学 阵列有助于形成30 nm染色质纤维和更高 染色体结构域，折叠提供了一个屏障，RNA 聚合酶可以被其他蛋白质如H1修饰， 转录活性的特定状态。 这些研究预计 提供必要的基线，以实现组装的目标， 转录活性和抑制的染色质状态在体外从 纯成分。

DESCRIPTION: The first level of DNA organization in eukaryotes resides in the nucleosome, which consists of approximately 200 bp of DNA wrapped around the histone octamer. The nucleosome is regarded as the most fundamental element of chromosomal structure. Incorporation of other proteins into oligonucleosomal arrays gives rise to specific chromatin structures capable of specific nuclear functions such as transcriptional activity. The general goals of the proposed research are to identify and characterize the structural and functional states of nucleosomal arrays and to understand the role of histones, linker histones, and transcription factors in directing and maintaining higher order chromatin structures brought about by nucleosome array folding and self- association. Three specific tasks are proposed to address the hypothesis that the intrinsic conformational dynamics of nucleosomal arrays contribute to formation of the 30 nm chromatin fiber and higher chromosomal domains, and that folding provides a barrier to RNA polymerases that can be modified by other proteins such as H1 to achieve specific states of transcriptional activity. These studies are expected to provide the baseline necessary to attain the goal of assembling transcriptionally active and repressed chromatin states in vitro from pure components.