CELL SORTER FACILITY

细胞分选设备

• 批准号: 3521497
• 负责人: BICE PERUSSIA
• 金额: $ 26.6万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-19 至 1993-06-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521497
• 关键词: biomedical equipment purchase; biomedical facility; flow cytometry; immunofluorescence technique

This proposal is to establish a multi-user flow cytometry facility at the Jefferson Cancer Institute, to support the research activities of several members of the Department of Microbiology and Immunology and of that of Medicine. To this purpose, we request funds to purchase a Coulter EPICS Elite flow cytofluorimeter. Currently, Thomas Jefferson University does not possess a facility of this type nor a cell sorter/analyzer dedicated to research activities. Two analyzers exist on campus: an Epics-C in the Division of Medicine Oncology, and a FACScan in the Hospital Clinical Pathology Laboratories. The Epics-C is not an up-to-date instrument, can not satisfactorily perform multicolor analysis, lacks U.V. capabilities, and can not be updated. The FACScan is dedicated to the Clinical Laboratory and can be used for research activities only as time permits. Neither machine has cell sorting capabilities. Flow cytometry technology has rapidly progressed and several techniques required by the scientific projects of our group require the EPICS Elite instrument. This instrument will allow us to establish a multi-user facility and will provide it with the ability to perform simple immunofluorescence experiments, as well as to sort cells with high purity and velocity, to perform 3 and 4 color immunofluorescence analysis, efficient use of dyes requiring U.V. activation (e.g. Indo-1 and Hoechst 33342), and simultaneous analysis of cell cycle by DNA staining and surface antigens by two-color immunofluorescence. We propose to use the EPICS Elite for a variety of projects, including cellular immunology, hematology, control of cell growth, and tumor biology.

该建议是在国家实验室建立一个多用户流式细胞仪设施， 杰斐逊癌症研究所，以支持研究活动的几个 微生物学和免疫学系的成员以及 药 为此，我们要求资金购买库尔特史诗 Elite流式细胞荧光计。 目前，托马斯杰斐逊大学 没有这种类型的设施，也没有专用的细胞分选仪/分析仪， 研究活动。 校园里有两台分析仪： 内科肿瘤学分部和医院临床中的FACScan 病理学实验室。 Epics-C不是最新的仪器，可以 不能令人满意地进行紫外线分析，缺乏紫外线能力， 并且不能被更新。 FACScan专用于临床 实验室，只有在时间允许的情况下才能用于研究活动。 这两台机器都没有细胞分选能力。 流式细胞术技术 已经取得了迅速的进展，科学研究所需要的几种技术 我们集团的项目需要EPICS Elite仪器。 本文书 将使我们能够建立一个多用户设施，并将为它提供 进行简单免疫荧光实验的能力，以及 以高纯度和高速度分选细胞，进行3色和4色 免疫荧光分析，需要紫外线的染料的有效使用。 激活（例如Indo-1和Hoechst 33342），并同时分析 DNA染色法检测细胞周期，双色法检测表面抗原 免疫荧光 我们建议将EPICS Elite用于各种 项目，包括细胞免疫学，血液学，细胞控制 生长和肿瘤生物学。