CELL SORTER FACILITY

这项建议是建立一个多用户流式细胞仪设施 杰斐逊癌症研究所，支持几项研究活动 微生物学和免疫学系成员以及 医学。为此，我们请求资金购买一部库尔特史诗 精英流细胞荧光仪。目前，托马斯·杰斐逊大学有 没有这种类型的设施，也没有专门的细胞分选器/分析仪 开展研究活动。校园里有两个分析器：史诗中的C 内科肿瘤科与FACScan在医院的临床 病理学实验室。EPICS-C不是最新的乐器，可以 多色分析不理想，缺乏紫外光能力， 并且不能更新。FACScan致力于临床 实验室，只有在时间允许的情况下才能用于研究活动。 这两台机器都没有细胞分选能力。流式细胞仪技术 已经取得了快速的进步，科学上需要的几种技术 我们组的项目需要史诗精英乐器。这台仪器 将使我们能够建立一个多用户设施，并将为其提供 进行简单的免疫荧光实验的能力，以及 以高纯度和高速度分选细胞，进行3色和4色 免疫荧光分析，高效使用需要UV的染料。 激活(例如INDO-1和HOECHST 33342)，并同时分析 细胞周期DNA染色和表面抗原双色法 免疫荧光。我们建议将史诗精英用于各种不同的 项目，包括细胞免疫学、血液学、细胞控制 生长和肿瘤生物学。

This proposal is to establish a multi-user flow cytometry facility at the Jefferson Cancer Institute, to support the research activities of several members of the Department of Microbiology and Immunology and of that of Medicine. To this purpose, we request funds to purchase a Coulter EPICS Elite flow cytofluorimeter. Currently, Thomas Jefferson University does not possess a facility of this type nor a cell sorter/analyzer dedicated to research activities. Two analyzers exist on campus: an Epics-C in the Division of Medicine Oncology, and a FACScan in the Hospital Clinical Pathology Laboratories. The Epics-C is not an up-to-date instrument, can not satisfactorily perform multicolor analysis, lacks U.V. capabilities, and can not be updated. The FACScan is dedicated to the Clinical Laboratory and can be used for research activities only as time permits. Neither machine has cell sorting capabilities. Flow cytometry technology has rapidly progressed and several techniques required by the scientific projects of our group require the EPICS Elite instrument. This instrument will allow us to establish a multi-user facility and will provide it with the ability to perform simple immunofluorescence experiments, as well as to sort cells with high purity and velocity, to perform 3 and 4 color immunofluorescence analysis, efficient use of dyes requiring U.V. activation (e.g. Indo-1 and Hoechst 33342), and simultaneous analysis of cell cycle by DNA staining and surface antigens by two-color immunofluorescence. We propose to use the EPICS Elite for a variety of projects, including cellular immunology, hematology, control of cell growth, and tumor biology.