FLOW CYTOMETRY FACILITY HIGH SPEED SORTER

描述（由申请人提供）：本提案旨在获得一台具有分选功能的最先进流式细胞仪，以升级托马斯杰斐逊大学Kimmel癌症中心的多用户流式细胞仪，从而为癌症中心NIH资助成员的研究活动提供最佳支持。我们请求购买Cytomation MoFlo（TM）HTS超高速分选流式细胞仪，该流式细胞仪具有高速4路分选能力，能够对4种以上的荧光染料进行常规同时分析，并具有单细胞沉积的附加能力。目前，KCC流式细胞仪设施是TJU校园内唯一的此类设施。它配备了一台12年的Coulter EPICS Elite ESP（也是一台分选仪器）和两台分析仪：一台4年的Coulter EPICS XL-MCL和一台15年的Becton-Dickinson FACSCalibur台式分析仪。TJU神经科实际上有一台新购置的BD Aria分类器（尽管尚未安装在新开发的设施中）。然而，无论何时运行，这台设备都将致力于满足神经科学研究的特定需求，KCC研究人员的访问权限有限。越来越多的KCC研究者在其NIH赞助的项目中需要高速细胞分选，这是我们在现有和运营的KCC设施中要求Cytomation MoFlo的主要理由。我们的申请集中在该核心设施升级其服务的需求上，这是由于：1）我们的NIH研究者越来越多地要求在合理的时间段内分选样品的期望的细胞产量，这是目前用现有设备无法实现的; 2）越来越多地要求在大细胞群体内进行稀有事件分选，以及同时分析多于4个荧光染料可检测的参数;以及3）缺乏对附近的高速分拣服务的足够和可靠的访问。所要求的Cytomation MoFlo仪器的可用性将允许在KCC继续进行杰出的基础和临床研究，并启动新项目。它还将提高现有设施的效率，使更多的调查人员能够可靠地使用这些仪器，并使其他调查人员（不需要这里所要求的机器）能够及时地对旧设备进行更常规的分析和分类，而不会出现目前的滞后现象。

DESCRIPTION (provided by applicant): This proposal is to acquire a state-of-the-art flow cytometer with sorting capabilities in order to upgrade the multi-user Flow Cytometry Facility of the Kimmel Cancer Center, Thomas Jefferson University, and thus optimally support the research activities of the NIH-funded members of the Cancer Center. We request to purchase a Cytomation MoFlo(TM) HTS Ultra High-Speed Sort Flow Cytometer with capability of high speed 4-way sorting, of routine simultaneous analysis of greater than 4 fluorochromes, and with the added capability of single cell deposition. Currently, the KCC Flow Cytometry Facility is the sole facility of this type on the TJU campus. It is equipped with a 12-year old Coulter EPICS Elite ESP, which is also a sorting instrument, and two analyzers: a 4-year old Coulter EPICS XL-MCL, and a 15- year old Becton-Dickinson FACSCalibur Benchtop Analyzer. A newly acquired BD Aria Sorter is physically present (although yet to be installed in a newly to-be-developed facility) in the Neurology Department at TJU. Whenever in operation, however, this piece of equipment will be dedicated to the specific needs of Neurological Sciences research, with the KCC investigator having limited access. The increased number of KCC investigators requiring high-speed cell sorting in their NIH-sponsored projects serves as the main justification for our request for a Cytomation MoFlo in the existing and operating KCC facility. Our application is focused on the need of this core facility to upgrade its services as a result of: 1) our NIH-investigators' increasing requests for desirable cell yields of sorted samples in reasonable time periods, which are currently unachievable with the present equipment; 2) an increasing demand for rare-event sorting within large cell populations, and for simultaneous analysis of greater than 4 fluorochrome-detectable parameters; and 3) the lack of sufficient and reliable access to near-by high speed sorting services. The availability of the Cytomation MoFlo instrument requested would allow outstanding basic and clinical research to be continued, and new projects to be initiated, at the KCC. It also would increase the efficiency of the existing Facility, allowing reliable use of the instruments by more investigators, and by other investigators (not in need of the machine requested here) to perform the more routine analysis and sorting in a timely manner on the old equipment, without the lag existent at present.