FLOW CYTOMETRY FACILITY HIGH SPEED SORTER

流式细胞术设备高速分选机

• 批准号: 6877619
• 负责人: BICE PERUSSIA
• 金额: $ 25.67万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-15 至 2006-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877619
• 关键词: biomedical equipment purchase; cell biology; cytology; flow cytometry

DESCRIPTION (provided by applicant): This proposal is to acquire a state-of-the-art flow cytometer with sorting capabilities in order to upgrade the multi-user Flow Cytometry Facility of the Kimmel Cancer Center, Thomas Jefferson University, and thus optimally support the research activities of the NIH-funded members of the Cancer Center. We request to purchase a Cytomation MoFlo(TM) HTS Ultra High-Speed Sort Flow Cytometer with capability of high speed 4-way sorting, of routine simultaneous analysis of greater than 4 fluorochromes, and with the added capability of single cell deposition. Currently, the KCC Flow Cytometry Facility is the sole facility of this type on the TJU campus. It is equipped with a 12-year old Coulter EPICS Elite ESP, which is also a sorting instrument, and two analyzers: a 4-year old Coulter EPICS XL-MCL, and a 15- year old Becton-Dickinson FACSCalibur Benchtop Analyzer. A newly acquired BD Aria Sorter is physically present (although yet to be installed in a newly to-be-developed facility) in the Neurology Department at TJU. Whenever in operation, however, this piece of equipment will be dedicated to the specific needs of Neurological Sciences research, with the KCC investigator having limited access. The increased number of KCC investigators requiring high-speed cell sorting in their NIH-sponsored projects serves as the main justification for our request for a Cytomation MoFlo in the existing and operating KCC facility. Our application is focused on the need of this core facility to upgrade its services as a result of: 1) our NIH-investigators' increasing requests for desirable cell yields of sorted samples in reasonable time periods, which are currently unachievable with the present equipment; 2) an increasing demand for rare-event sorting within large cell populations, and for simultaneous analysis of greater than 4 fluorochrome-detectable parameters; and 3) the lack of sufficient and reliable access to near-by high speed sorting services. The availability of the Cytomation MoFlo instrument requested would allow outstanding basic and clinical research to be continued, and new projects to be initiated, at the KCC. It also would increase the efficiency of the existing Facility, allowing reliable use of the instruments by more investigators, and by other investigators (not in need of the machine requested here) to perform the more routine analysis and sorting in a timely manner on the old equipment, without the lag existent at present.

描述(由申请人提供)：这项建议是为了获得一台最先进的具有分选能力的流式细胞仪，以升级托马斯·杰斐逊大学基梅尔癌症中心的多用户流式细胞仪设备，从而以最佳方式支持NIH资助的癌症中心成员的研究活动。我们要求购买一台Cytomation MoFlo(TM)HTS超高速分选流式细胞仪，具有高速四向分选的能力，可同时分析4种以上的荧光染料，并具有附加的单细胞沉积能力。目前，KCC流式细胞术设备是TJU校园内唯一的此类设备。它配备了有12年历史的Coulter EPICS Elite ESP，这也是一个分类仪器，以及两个分析仪：有4年历史的Coulter EPics XL-MCL和有15年历史的Becton-Dickinson FACSCalibur Benchtop Analyzer。新收购的BD Aria Sorter在TJU神经科实际存在(尽管尚未安装在新开发的设施中)。然而，无论何时运行，这台设备都将专门用于神经科学研究的特定需求，而KCC的研究人员只能有限地访问。越来越多的KCC调查人员在他们的NIH赞助的项目中需要高速细胞分选，这是我们要求在现有和运行的KCC设施中进行细胞切割MoFlo的主要理由。我们的应用重点关注这一核心设施升级其服务的需求，原因是：1)我们的NIH调查人员越来越多地要求在合理的时间段内获得理想的分选样本细胞产量，这是目前使用现有设备无法实现的；2)对大型细胞群体中的罕见事件分选以及对超过4个可检测荧光色素的参数的同时分析的需求日益增长；以及3)缺乏足够和可靠的近距离高速分选服务。所要求的Cytomation MoFlo仪器的供应将使KCC能够继续进行杰出的基础和临床研究，并启动新的项目。它还将提高现有设施的效率，使更多的调查员和其他调查员(不需要这里要求的机器)可靠地使用仪器，以便及时对旧设备进行更常规的分析和分类，而不会出现目前存在的滞后现象。