EPICS-C CYTOFLUOROMETRY FOR STUDIES IN IMMUNOBIOLOGY

我们建议购买并操作EPICS-C细胞荧光仪/细胞分选机 配备Tektronics 4612录像机的系统，目的是追求更多 我们正在进行的关于免疫调节性淋巴亚群的研究 与移植有关的各种疾病状态和动物模型， 自身免疫性疾病和急性烧伤。核心用户组项目a 需要80%-100%的机器使用率。我们都参与了NIH资助的研究 随着对这种仪器的需求不断增长，而这是无法通过 该地区现有的仪器设备。我们集团有一段历史， 合作的努力，我们大多数人现在都处于相同的位置 布里格姆妇女医院的检验区。所选的乐器 将允许我们所需的大规模分析和细胞分选 协议。有时，某些实验可能需要双激光器 机器，但这在有限的基础上在FACS440上提供 在这所医院里。

We propose to acquire and operate an EPICS-C cytofluorometer/cell sorter system with a Tektronics 4612 video copier for the purpose of pursuing more efficiently our ongoing studies of immunoregulatory lymphoid subsets in a variety of disease states and animal models relating to transplantation, autoimmune disease, and acute burn injury. The core user's group project a need for 80-100% machine usage. We are all engaged in NIH funded research with a growing need for this instrumentation, and this cannot be met by existing instrumentation in the area. Our group has a history of cooperative endeavors, and most of us are now located in the same laboratory area in the Brigham and Women's Hospital. The instrument chosen will permit the largescale analysis and cell sorting needed by our protocols. On occasion, certain experiments may require a dual laser machine, but this is available on a limited basis on the FACS 440 located in this hospital.