INNER EAR ULTRASTRUCTURE AND PATHOLOGY

这是对具有能量的高分辨率电子显微镜的要求 色散X射线显微分析支持协调的超微结构 对内耳的检查。重点将放在耳蜗上。 膜迷路，并特别注意了解 听力障碍的原因及人工耳蜗术的应用。 这套仪器将补充美国国家航空航天局的研究设施 耳鼻咽喉科和KHRI，并将创建一个独特的中心 为超微结构研究耳蜗病理学和临床 人工耳蜗术的应用形态分析是一个点 分析和实验研究的融合采取了解剖学、 行为、生化、电生理方法来阐明 感音神经性耳聋的机制及耳蜗术中的作用 假体在帮助听力受损人士中的作用。人类和人类的使用 平行的动物组织应该导致对其有用性的确定 目前用于研究听力功能的动物模型。 对高分辨率电子显微镜的需求是基于 以下是正在进行的和即将进行的NIH赠款支持的具体目标：1) 暴露后内耳组织超微结构观察 耳毒性药物(利尿剂、抗生素、抗肿瘤药物)和 强烈的噪音。2)检测重金属，例如抗肿瘤药物 (顺铂)在内耳细胞内的分布。3)研究 内耳屏障受压时紧密连接的结构完整性 由噪音、耳毒剂和内耳疾病引起。4)检查内耳 给予耳毒性药物、噪声和改变后的血管构筑 微动态平衡条件。5)识别人体内的病毒颗粒 耳朵标本。6)检测病毒诱导的超微结构效应 耳蜗学和前庭病理学。7)超微结构形态检查 培养的上皮性和内皮内耳细胞。8)衡量标准 培养的内皮细胞和内皮细胞中的电解质 耳朵。9)检查人工电极在体内的超微结构效应 耳蜗管。10)检测假体电极的心理离散度 耳蜗组织。

This is a request for a high resolution electron microscope with energy dispersive x-ray microanalysis to support coordinated, ultrastructural investigations of the inner ear. The focus will be on the cochlear membranous labyrinth, with specific attention given to understanding of the causes of hearing impairment and the application of cochlear prosthesis. This instrumentation package will complement the research facilities of the Department of Otorhinolaryngology and KHRI and will create a unique center for ultrastructural investigations into cochlear pathology and the clinical use of cochlear prosthesis. Morphological analysis is a point of convergence of analytical and experimental studies taking an anatomical, behavioral, biochemical, electrophysiological approach to illuminate mechanisms underlying sensorineural hearing loss and the role of cochlear prosthesis in aiding hearing impaired individuals. The use of human and animal tissue in parallel should lead to a determination of the usefulness of animal models now employed for studies of hearing function. The need for a high resolution electron microscope is based upon the following specific aims of active and pending NIH grant support: 1) Examine ultrastructural features of the inner ear tissues after exposures to ototoxic agents (diuretics, antibiotics, antineoplastic agents) and to intense noise. 2) Detect heavy metals, e.g., antineoplastic agents (cis-platinum) distribution within inner ear cells. 3) Study the structural integrity of tight junctions of inner ear barriers when stressed by noise, ototoxic agents, and inner ear disease. 4) Examine the inner ear vasculature after administration of ototoxic drugs, noise, and in altered microhomeostatic conditions. 5) Identify viral particles in human inner ear specimens. 6) Examine ultrastructural effects of viral-induced cochlear and vestibular pathology. 7) Examine ultrastructural morphology of cultured epithelial and endothelial inner ear cells. 8) Measure electrolytes in cultured epithelial and endothelial cells of the inner ear. 9) Examine ultrastructural effects of prosthetic electrodes placed in the cochlea. 10) Detect mental dispersion of prosthetic electrodes within the cochlear tissues.