PROTEIN ANALYSIS EQUIPMENT

建议购买一套专门仪器， 蛋白质/肽的纯化及其氨基酸的测定 序列的 该设备，其中包括氨基酸分析仪，气体 相序仪和制备型高效液相色谱仪， 将沿着已经投入使用的其他设备， 蛋白质/核酸分析实验室（P/NAAL），提供 向主要由首席调查员组成的用户组提供分析 以及加州大学欧文分校的多位同事。 这些将 范围从全氨基酸序列测定到合成 寡核苷酸探针/引物。 需要P/NAAL支助的项目 主要研究人员实验室包括对结构的研究， 几种NAD+依赖性辅酶A酶的生物合成， 突变型E.大肠杆菌碱性磷酸酶基因的序列分析 磷酸甘油酸酯，肝和肠凝集素，支持 牛精浆、豚鼠前列腺和响尾蛇亚基 神经生长因子和垂体成纤维细胞生长的氨基末端区域 因子 加州大学欧文分校的其他研究人员和 邻近的机构需要P/NAAL支持各种项目， 基因结构和表达，一方面， 另一方面是细胞表面受体 特别是，涉及 蛋白酶连接蛋白、丙酮酸激酶同工酶、天冬酰胺 合成酶，核糖体蛋白，细胞色素P-450 SCC，免疫球蛋白， 叶绿体醛缩酶和盐视紫红质的描述以及研究 处理蛋白质合成细菌铁运输 许多这些 项目还涉及基因拼接和核酸序列 决心。 此外，还有相当多的未成年人。 用户拥有同样广泛的项目。 这些的广度 项目形式的主要和次要用户强烈强调了许多 现代蛋白质化学分析方法的应用及应用价值 提供此类服务的中心实验室。

It is proposed to purchase a set of specialized instruments for the purification of proteins/peptides and the determination of their amino acid sequences. This equipment, which includes an amino acid analyzer, gas phase sequencer and preparative high performance liquid chromatograph, will, along with additional equipment already in operation as part of the Protein/Nucleic Acid Analysis Laboratory (P/NAAL), provide a spectrum of analyses to the users group, composed mainly of the principal investigator and various colleagues at the University of California-Irvine. These will range from full amino acid sequence determinations to the synthesis of oligonucleotide probes/primers. Projects requiring P/NAAL support from the principal investigators laboratory include studies on the structure and biosynthesis of several NAD+ dependent dehydrogenases, characterization of mutant forms of E. coli alkaline phosphatase, and the sequence analyses of phosphoglycerate mutase, liver and intestinal lectins, the supporting subunits of bovine seminal plasma, guinea pig prostate and rattlesnake NGF's, and the amino terminal region of pituitary fibroblast growth factor. Other investigators at the University of California-Irvine and neighboring institutions require P/NAAL support for projects as diverse as gene structure and expression, on the one hand, and characterization of cell surface receptors on the other. In particular, projects involving analyses of protease nexins, pyruvate kinase isozymes, asparagine synthetase, ribosomal proteins, cytochrome p-450scc, immunoglobins, chloroplast aldolases and halorhodopsin are described as well as studies dealing with protein synthesis, bacterial iron transport. Many of these projects also involve gene splicing and nucleic acid sequence determinations. In addition, there are a considerable number of minor users with an equally broad variety of projects. The breadth of these projects form both major and minor users strongly underscores the many applications of modern protein chemical analyses and the value of a centralized laboratory for providing such services.