HIGH SENSITIVITY PROTEIN ISOLATION/ANALYTICAL SYSTEM

建议购买一套指定仪器， 分离、序列和组成分析 蛋白质/肽。 该设备包括在线PTH 现有470 A气相测序仪的分析/数据系统，高 灵敏度氨基酸分析仪，微孔HPLC蛋白质/ 肽分离和现有肽的更新， 寡核苷酸合成器。 这些额外的项目将使 蛋白质/核酸分析实验室（P/NAAL）提供 为多个用户提供增强的功能，使他们能够 继续进行目前正在进行的广泛研究。 的 这一要求的重点是提供一个平衡和 一套协调的分析工具，使三个 组分分离、氨基酸分析和序列分析 是互补的。 如果任何一个领域是薄弱的，那么加强 其他领域的能力都被浪费了。 项目要求 主要研究者的P/NAAL支持包括以下研究： 苹果酸脱氢酶的功能， 猪精浆酶，牛精浆的支持亚单位， 豚鼠前列腺和响尾蛇神经生长因子的分离， 蛋氨酸氨肽酶和N- 乙酰转移酶 其他研究人员在大学的 加州-欧文需要P/NAAL支持各种各样的 项目 特别是涉及蛋白酶分析的项目 连接蛋白，细胞色素p-450，蛋白质羧甲基转移酶，和 受精蛋白结合。 此外，本发明还提供了一种方法， 有相当数量的未成年人用户， 各种各样的利益。 这些项目的气息来自两大 和小用户强烈强调了许多应用程序， 现代蛋白质/核酸分析和集中的价值 实验室提供这些服务。

It is proposed to purchase a set of specified instruments for the isolation, sequence and compositional analysis of proteins/peptides. This equipment includes an online PTH analysis/data system for an existing 470A Gas Phase Sequencer, high sensitivity amino acid analyzer, microbore HPLC for protein/ peptide isolation and updates for the existing peptide and oligonucleotide synthesizers. These additional items will enable the Protein/Nucleic Acid Analysis Laboratory (P/NAAL) to provide enhanced capabilities to its multiple users and enable them to continue with the broad range of research currently underway. The particular focus of this request is to provide a balanced and coordinated set of analytical tools that will enable the three components of isolation, amino acid analysis and sequence analysis to be complementary. If any one area is weak, then enhanced capabilities in the other areas are wasted. Projects requiring P/NAAL support from the principal investigators include studies on the function of malate dehydrogenases, the sequence analyses of porcine fumarase, the supporting subunits of bovine seminal plasma, guinea pig prostate and rattlesnake NGF's and the isolation, characterization and cloning of methionine aminopeptidase and N- acetyl transferase. Other investigators at the University of California-Irvine require P/NAAL support for a diverse range of projects. In particular, projects involving analyses of protease nexins, cytochrome p-450's, protein carboxymethyltransferases, and the fertilization proteins binding, are described. In addition, there are considerable number of minor users with an equally broad variety of interests. The breath of these projects from both major and minor users strongly underscores the many applications of modern protein/nucleic analyses and the value of a centralized laboratory for providing such services.