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PURCHASE OF A HIGH FIELD NMR SPECTROMETER

购买高场核磁共振波谱仪

基本信息

批准号：
3519203
负责人：
JULES Alan SHAFER
金额：
$ 30万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
1985
资助国家：
美国
起止时间：
1985-01-01 至 1986-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3519203
关键词：
biochemistry carbohydrate structure nuclear magnetic resonance spectroscopy

项目摘要

Funds are requested for purchase of a high field NMR spectrometer for observation of 1H and 13C nuclei to determine structures of complex low molecular weight compounds (Mr Less than 6,000) and to characterize protein ligand interactions. Studies are proposed directed toward determination of the possible relationship between the structure of the carbohydrate side chains of human fibrinogen and its concentration in plasma in individuals with normal and inherited abnormal fibrinogens. The NMR will also be used to determine the structure of glycopeptides obtained from cell surface proteins, as well as to determine the structure of laboratory synthesized cofactors, cofactor analogs, oligosaccharides, and substrates of enzymically catalyzed reactions. Attempts will be made to determine the ionization behavior of imidazole and other groups in peptides and proteins from measurements of the pH dependence of 1H and 13C resonances. Some of these studies are directed toward characterization of imidazolium thiolate ion pair interactions which appear to be important for the activity of several proteins. Additionally the instrument will be used to determine thermodynamic and kinetic parameters for the binding of carbohydrates to lectins. Nuclear Overhauser effects and transfer of nuclear Overhauser effects will be studied in attempts to characterize alterations in ligand conformation upon their binding to protein, the relative disposition of FAD and NAD+, in lipoamide dehydrogenase and glutathione reductase, as well as to determine the orientation of enzyme bound substrate analogs with respect to pyridoxal phosphate in D-serine dehydratase. Attempts also will be made to determine the structure of stabilized enzyme bound cofactor intermediates in an effort to establish reaction pathways for folate, pyridoxal phosphate and flavin requi0ing enzymes. Additionally the spectrometer will be used to determine the structure of products and monitor the progress of enzymically catalyzed reactions where other methods of analysis are not feasible.

请求提供资金，用于购买一台高场核磁共振分光计，用~ 1H和~（13）C原子核测定络合物低结构分子量化合物（Mr小于6，000）和表征蛋白质配体相互作用建议进行研究，以确定碳水化合物侧的结构之间的可能关系人纤维蛋白原链及其在个体血浆中的浓度正常和遗传性异常纤维蛋白原。核磁共振也将用于确定从细胞表面获得的糖肽的结构蛋白质，以及确定实验室合成的结构辅因子、辅因子类似物、寡糖和底物酶催化反应。将尝试确定多肽和蛋白质中咪唑等基团电离行为来自1H和13 C共振的pH依赖性的测量。一些这些研究针对咪唑硫醇盐的表征，离子对相互作用，这似乎是重要的活动，几种蛋白质。此外，该仪器将用于确定热力学和动力学参数的碳水化合物结合，凝集素。核奥弗豪泽效应与核奥弗豪泽转移将研究影响，试图表征配体的改变，与蛋白质结合后的构象、FAD的相对分布和NAD+，硫辛酰胺脱氢酶和谷胱甘肽还原酶，以及为了确定酶结合底物类似物相对于磷酸吡哆醛在D-丝氨酸脱氢酶中的作用。我们也会尝试确定稳定的酶结合辅因子的结构中间体在建立叶酸的反应途径的努力中，磷酸吡哆醛和黄素需要酶。另外光谱仪将用于确定产品的结构，监测酶催化反应的进展，分析是不可行的。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Self-regulatory fatigue, quality of life, health behaviors, and coping in patients with hematologic malignancies.

血液系统恶性肿瘤患者的自我调节疲劳、生活质量、健康行为和应对。

DOI：
10.1007/s12160-014-9621-z
发表时间：
2014
期刊：
Annals of behavioral medicine : a publication of the Society of Behavioral Medicine
影响因子：
0
作者：
SolbergNes,Lise;Ehlers,ShawnaL;Patten,ChristiA;Gastineau,DennisA
通讯作者：
Gastineau,DennisA

Relaxin suppresses atrial fibrillation in aged rats by reversing fibrosis and upregulating Na+ channels.

DOI：
10.1016/j.hrthm.2015.12.030
发表时间：
2016-04
期刊：
Heart rhythm
影响因子：
5.5
作者：
Henry BL;Gabris B;Li Q;Martin B;Giannini M;Parikh A;Patel D;Haney J;Schwartzman DS;Shroff SG;Salama G
通讯作者：
Salama G

19F NMR studies on 8-fluoroflavins and 8-fluoro flavoproteins.

8-氟黄素和 8-氟黄素蛋白的 19F NMR 研究。