FLUORESCENT ACTIVATED CELL SORTER UPGRADE SYSTEM

这项建议的目的是更新由 南加州大学的流式细胞术核心设施。从1980年起，贝克顿- Dickinson FACS IV已经向南加州大学的成员提供 癌症中心。该服务已被15个NIH支持的用户使用 癌症中心的成员，他们参与了基础和 临床调查。FACS用于分析和 通过细胞分选、DNA和/或RNA分析进行纯化，以及 包括细胞周期分析在内的各种双色程序 特定的淋巴细胞亚群。我们要求更换 破旧的设备和新的改装已经成为 从1980年开始供应。这台设备包括一台新型激光器，一台 电脑，和一个冷却系统，以保存昂贵的寿命 激光。有了这台设备，我们将改进我们的图形 数据的演示，我们将能够执行三个 表面标记的颜色分析，这一程序将使 美国将更精确地定义功能特异的淋巴细胞 子集，并更精确地分析特定的细胞周期 淋巴细胞亚群。这种新设备将使我们能够 根据要求灵活地开发流式细胞仪的新用途 由我们现在和未来的用户提供。

The purpose of this proposal is to update the service provided by the USC Flow Cytometry Core Facility. Since 1980 a Becton- Dickinson FACS IV has been available to members of the USC Cancer Center. This service has been used by 15 NIH supported members of the Cancer Center who are involved in basic and clinical investigations. The FACS is used for analysis and purification by cell sorting, DNA and/or RNA analysis, and various two color procedures including cell cycle analysis of specific lymphocyte subsets. We are requesting replacement of worn out equipment and new modifications which have become available since 1980. This equipment consists of new lasers, a computer, and a cooling system to preserve the life of expensive lasers. With this equipment we will improve our graphic presentation of the data and we will be able to perform three color analysis of surface markers, a procedure which will enable us to define more precisely functionally specific lymphocyte subsets, and to analyze more precisely the cell cycle of specific lymphocyte subsets. This new equipment will allow us the flexibility to develop new uses for the flow cytometer as dictated by our current and future users.