Flow Cytometer

描述（由申请人提供）：流式细胞术核心设施具有 作为前沿生物医学研究的主要资源， 由美国国立卫生研究院资助的南加州大学/诺里斯综合癌症中心的成员， 过去15年这项拨款的目的是更换现有的细胞分选机 1987年获得的，制造商已宣布将在 2001年夏天。在此时间之后，制造商无法保证零件或 现在广泛使用的荧光色素的产物作为 单克隆抗体，特别是用于四聚体染色。此外，本发明还提供了一种方法， 三色分析对我们的许多研究人员来说已经不够用了。的 更换后的仪器将提供5色甚至6色分析 识别和分选稀有细胞的能力，以及新的 仪器将得到极大的改进。这一新工具将使我们能够 灵活性，以开发新的用途，为流式细胞仪所规定的，我们的 现在和未来的用户。

DESCRIPTION (provided by applicant): The Flow Cytometry Core Facility has served as the principal resource for cutting edge biomedical research performed by members of the NIH-funded USC/Norris Comprehensive Cancer Center for the past 15 years. The purpose of this grant is to replace the present cell sorter obtained in 1987 which the manufacturer has declared will become obsolete in the summer of 2001. After this time the manufacturer cannot gaurantee parts or product s for the flourochhromes now in widespread usage as conjugates for monoclonal antibodies, especially for tetramer staining. In addition, three-color analysis is no longer adequate for many of our investigators. The replacement instrument will provide 5 and even 6-color analysis for the purpose of identifying and sorting rare cells, and the sorting efficiency of the new instrument will be greatly enhanced. This new instrument will allow us the flexibility to develop new uses for the flow cytometer as dictated by our current and future users.