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LCVD: Low-cost Cell-extract Viral Diagnostics

LCVD：低成本细胞提取物病毒诊断

基本信息

批准号：
EP/R014000/1
负责人：
James Ajioka
金额：
$ 196.35万
依托单位：
University of Cambridge
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=EP%2FR014000%2F1
关键词：
LCVD Low cost Cell extract

项目摘要

Virus disease burden in South AfricaSouth Africa has one of the highest rates of HIV infection worldwide. For infected individuals, drug therapy depends on knowing their virus load (VL) that is, how much virus is in circulation. About 2.4 million HIV VL tests costing $25 to $40 each, were performed last year in SA but the test is still unavailable those without access to major health care centres. Human norovirus (HuNoV; "winter vomiting virus") is highly infectious and responsible for 90% of non-bacterial diarrheal disease and studies in sub-Saharan Africa suggest that a significant proportion of diarrhea-related deaths in children under 5 go unreported. Thus it is likely that HuNoV infection is an underestimated health threat in SA. Addressing both of these problems with low cost point-of care diagnostic tests would greatly improve diagnosis and treatment, reducing mortality and also the heavy economic burden these diseases.Aims and goals of the proposalOur ultimate aim is to co-develop methods to rapidly design and construct cost-effective point-of-care diagnostics for infectious disease agents responsible for high morbidity and mortality in South Africa and other low-income regions. We will target HIV virus load (VL) testing and human norovirus (HuNoV) diagnosis. Recently, biologically-based "biosensor" tests for Zika virus and Ebola virus have been developed that can be produced on paper strips. These tests have the potential to be very low cost because they use a soup or extract from cells to identify the virus. The "cell-free" extract can be spotted onto paper strips, which allows a type of "dipstick" biosensor test for the virus. We plan to refine this basic process by having a team of UK and SA researchers co-develop the cell-free biosensors for HIV and HuNoV. The co-development will both train SA researchers in method development but also allow the method to be taught to other researchers that can then apply the method to their own research. Hence, we aim to co-develop a sustainable technology for SA and other low-income areas. Our goals are: 1) to produce prototype low cost point-of-care diagnostic tests for HIV VL testing and HuNoV diagnosis 2) to use the co-development research process to generate a set of cell-free biosensor methods as a platform technology accessible to SA and other LMIC researcher and educators 3) to use the co-development research process to broadly train individual researchers (our research team) and teach the methods and train other researchers in SA. Our proposal is based on co-development of cell-free in vitro biosensor diagnostics using our research teamwork to drive innovation and integration of laboratories and personnel in the UK and South Africa.

南非的病毒疾病负担南非是世界上艾滋病毒感染率最高的国家之一。对于受感染的个体，药物治疗取决于知道他们的病毒载量（VL），即有多少病毒在循环中。去年在南非进行了大约240万次艾滋病毒VL检测，每次检测费用为25至40美元，但那些无法进入主要保健中心的人仍然无法获得这种检测。人诺如病毒（HuNoV;“冬季呕吐病毒”）具有高度传染性，并且是90%的非细菌性腹泻病的原因，并且在撒哈拉以南非洲的研究表明，在5岁以下儿童中，与腹泻相关的死亡中有很大一部分没有报告。因此，HuNoV感染可能是SA中被低估的健康威胁。解决这两个问题与低成本的护理点诊断测试将大大提高诊断和治疗，降低死亡率，也沉重的经济负担，这些diseases.Aims和目标的proposalOur的最终目的是共同开发的方法，以快速设计和构建成本效益的护理点诊断传染病病原体负责高发病率和死亡率在南非和其他低收入地区。我们将针对HIV病毒载量（VL）检测和人类诺如病毒（HuNoV）诊断。最近，针对寨卡病毒和埃博拉病毒的基于生物的“生物传感器”测试已经开发出来，可以在纸条上生产。这些测试有可能是非常低的成本，因为他们使用汤或提取物从细胞来识别病毒。“无细胞”提取物可以点在纸条上，这允许一种类型的“试纸”生物传感器测试病毒。我们计划通过让英国和南非的研究人员共同开发HIV和HuNoV的无细胞生物传感器来完善这一基本过程。共同开发既可以培训SA研究人员的方法开发，也可以将该方法教授给其他研究人员，然后将该方法应用于自己的研究。因此，我们的目标是为南非和其他低收入地区共同开发可持续技术。我们的目标是：1）生产用于HIV VL检测和HuNoV诊断的原型低成本即时诊断检测2）使用共同开发研究过程生成一套无细胞生物传感器方法，作为SA和其他LMIC研究人员和教育工作者可访问的平台技术3）使用共同开发研究过程广泛培训个人研究人员（我们的研究团队），教的方法和培训其他研究人员在SA。我们的建议是基于共同开发无细胞体外生物传感器诊断，利用我们的研究团队推动英国和南非实验室和人员的创新和整合。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Transmission of SARS-CoV-2 by children to contacts in schools and households: a prospective cohort and environmental sampling study in London.

DOI：
10.1016/s2666-5247(22)00124-0
发表时间：
2022-11
期刊：
LANCET MICROBE
影响因子：
38.2
作者：
Cordery, Rebecca;Reeves, Lucy;Zhou, Jie;Rowan, Aileen;Watber, Patricia;Rosadas, Carolina;Crone, Michael;Starch, Marko;Freemont, Paul;Mosscrop, Lucy;Cowley, Alice;Zefent, Gina;Bisset, Kate;Le Blond, Holly;Regmi, Sadie;Buckingham, Christian;Junaideen, Ramlah;Abdulla, Nadia;Eliahoo, Joseph;Mindlin, Miranda;Lamagni, Theresa;Barclay, Wendy;Taylor, Graham P.;Sriskandan, Shiranee
通讯作者：
Sriskandan, Shiranee

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