A Coordinated Infrastructure for NMR in the Physical and Life Sciences: A 1 GHz Spectrometer at Birmingham

物理和生命科学中 NMR 的协调基础设施：伯明翰的 1 GHz 光谱仪

• 批准号: EP/R030030/1
• 负责人: Tim Softley
• 金额: $ 835.72万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FR030030%2F1
• 关键词: Coordinated; Infrastructure; NMR; Physical; Life

Birmingham houses and supports the Henry Wellcome Building for Biomolecular NMR (HWB-NMR), which since 2004 has provided a durable, state-of-the-art NMR service at 600, 800 and 900MHz for UK users. With support of the Wellcome Trust, the facility offers 70% of its available high-field NMR time free of charge to external users with high-quality projects and has expert support staff in place. HWB-NMR has hosted >50 research groups from laboratories across the UK, thus assuring UK leadership in ultra-high-field NMR and has regularly been part of EU access networks.The popularity and importance of this facility for the UK NMR community is well evidenced by recent reviews, which qualified its services as "the vital scientific resource in UK" and "an excellent national facility which is clearly heavily used"; the Wellcome Trust renewed funding of the HWB-NMR operations for the period 2018-2023.HWB-NMR proposes the addition of a 1GHz spectrometer, that will provide UK users with a unique line-up of NMR instruments covering 600, 800, 900MHz and 1GHz spectrometers, primarily for biomedical applications. By providing these services at one location, and with the combined support of research councils and the Wellcome Trust, a unique resource will be created that will put the UK NMR community at the forefront of NMR research, both nationally and internationally.Specific services in Birmingham will include tracer-based and real-time metabolism, for which we will offer access to micro-cryoprobes at 600, 800MHz and 1GHz for ultimate mass sensitivity and will develop NMR methods that facilitate this line of research.By forming a strategic alliance with the structural biology research at the University of Leicester (Prof Geerten Vuister, to be appointed as liaising visiting professor) we will also be able to offer specific expertise in NMR methodology, both in NMR pulse sequences and computational aspects (BBSRC funded), drug discovery and NMR software development via the CCPN project (MRC funded). With CCPNs 28 national and international partners and its extensive outreach programme, the 1GHz spectrometer will be firmly embedded in the NMR community. University-provided research support will particularly focus on methodology developments that benefit from the unique range of NMR instrumentation at HWB-NMR.The direct interactions between Birmingham and Leicester in highly advanced NMR technology also aim to further strengthen their existing efforts in equipment and expertise sharing, as exemplified by the recent successful establishment of a shared cryo-EM facility across the Midlands universities. Jointly, the NMR and EM techniques contribute to the goal of integrated structural biology approaches for studying the molecular mechanisms that underpin both normal and aberrant cellular functioning.

伯明翰拥有并支持亨利惠康生物分子核磁共振大楼（HWB-NMR），自2004年以来，该大楼为英国用户提供了600、800和900 MHz的耐用、最先进的核磁共振服务。在Wellcome Trust的支持下，该设施将其70%的可用高场NMR时间免费提供给具有高质量项目的外部用户，并拥有专家支持人员。HWB-NMR已经接待了来自英国各地实验室的超过50个研究小组，从而确保了英国在超高场NMR领域的领导地位，并定期成为欧盟接入网络的一部分。该设施对英国NMR界的普及和重要性得到了最近的评论的充分证明，该设施的服务被评为“英国重要的科学资源”和“一个优秀的国家设施，显然被大量使用”; Wellcome Trust为2018-2023年期间的HWB-NMR业务提供了新的资金。HWB-NMR提议增加一台1GHz光谱仪，这将为英国用户提供独特的NMR仪器阵容，涵盖600，800，900 MHz和1GHz光谱仪，主要用于生物医学应用。通过在一个地点提供这些服务，并与研究委员会和惠康信托基金会的联合支持，将创建一个独特的资源，使英国NMR社区在国内和国际上处于NMR研究的前沿。伯明翰的具体服务将包括基于示踪剂和实时代谢，为此，我们将提供600，800 MHz和1 GHz的最终质量灵敏度，并将开发NMR方法，促进这一系列的研究。通过与莱斯特大学的结构生物学研究形成战略联盟，（Geerten Vuister教授，将被任命为联络访问教授）我们还将能够提供NMR方法学方面的具体专业知识，在NMR脉冲序列和计算方面（BBSRC资助），药物发现和NMR软件开发通过CCPN项目（MRC资助）。凭借CCPN的28个国家和国际合作伙伴及其广泛的推广计划，1GHz光谱仪将牢固地嵌入NMR社区。伯明翰大学和莱斯特大学在先进的核磁共振技术方面的直接互动也旨在进一步加强他们在设备和专业知识共享方面的现有努力，最近在米德兰兹大学成功建立了共享的冷冻EM设施。联合，NMR和EM技术有助于研究支持正常和异常细胞功能的分子机制的综合结构生物学方法的目标。