STRATOSPHERIC OZONE DEPLETION AND INCREASED DNA DAMAGE

平流层臭氧的耗尽将导致太阳活动的增加 到达地球表面的紫外线(UVR)。类型 将会增加的UVR是所谓的UV-B辐射(280-320 NM)，已知具有生物破坏性。增加的 UVR的水平无疑将导致数量的增加 人类皮肤癌。基于之前动物的结果 致癌研究，应该有可能利用DNA损伤 作为用于流行病学研究的生化标记物 预测皮肤癌水平上升与下降相关 平流层臭氧水平。 从拟议的研究中获得的信息将是 在确定表皮DNA水平增加时必不可少的 不同皮肤类型的人的损害作为一种 考虑到臭氧的消耗。具体来说，我们希望确定：1) UV-B区波长对DNA的诱导效应 I-V型皮肤者的皮肤损害按以下分类 对太阳的敏感度和晒黑反应；以及，2)使用太阳能 模拟器用于模拟与各种类型的UVR相关的传输 以确定臭氧消耗的水平是否为行动光谱 在目标1中产生的，实际上可以用来预测增加 臭氧将导致的DNA损伤程度 耗尽。

Depletion of stratospheric ozone will lead to an increase in solar ultraviolet radiation (UVR) reaching the Earth's surface. The type of UVR that will increase is the so-called UV-B radiation (280-320 nm) which is known to be biologically damaging. The increased levels of UVR will undoubtedly result in an increase in the number of human skin cancers. Based on results of previous animal carcinogenesis studies, it should be possible to use DNA damage as a biochemical marker for use in epidemiological studies to predict increased levels of skin cancer associated with decreased levels of stratospheric ozone. The information obtained from the proposed research will be essential in determining increased levels of epidermal DNA damage in individuals of various skin types as a function of a given ozone depletion. Specifically, we wish to determine: 1) the effectiveness of wavelengths in the UV-B region in inducing DNA damage in the skin of individuals of skin types I-V as classified by sensitivity to the sun and tanning response; and, 2) use solar simulators to mimic UVR transmission associated with various levels of ozone depletion to determine whether the action spectra generated in aim 1 can, in fact, be used to predict increased levels of DNA damage that will occur as a result of ozone depletion.