MONITORING HUMAN EXPOSURE TO AFLATOXINS IN THE GAMBIA

黄曲霉毒素B1（AFB 1）是一种对肝脏有很强毒性的毒素， 实验动物的致癌物质 这种化学物质也 存在于环境中，由于霉菌相关 食品和商品的腐败。 这些食物的消费 导致生活在许多地区的人摄入高AFB 1 亚洲和非洲。 许多研究人员使用经典的 流行病学方法来检查可能的因果关系 饮食中的黄曲霉毒素B1和肝癌之间的关系 而强 AFB 1暴露与肝癌之间的关联已经被 新技术直到最近才出现， 监测个别人士接触黄曲霉毒素B1的情况和代谢情况， 人 这些方法将有助于解剖的作用 病毒因子，如B型肝炎，从黄曲霉毒素在 肝癌的病因。 生物标志物的发展， 评估黄曲霉毒素的暴露将有助于限制 人群中的潜在风险。 在下文中， 研究中，我们将研究季节和年度变化， 黄曲霉毒素摄入、代谢、尿和白蛋白处置 生活在西非冈比亚的人， 患上肝癌 这些研究将系统地探讨 从膳食中摄入的黄曲霉毒素与生物标志物，如 排泄的AFB-DNA加合物，尿中氧化代谢产物，黄曲霉毒素 ml并将黄曲霉毒素共价结合到血清白蛋白上， 为了确定这些标记物中的哪一个对于非侵入性地 评估肝脏高风险人群的暴露状况 癌

Aflatoxin Bl (AFBl) is one of the most potent liver carcinogens known for experimental animals. This chemical is also present in the environment as a result of mold related spoilage of foods and commodities. Consumption of these foods results in high intake of AFBl by people living in many regions of Asia and Africa. A number of researchers have used classic epidemiological methods to examine the possible causal relationship between AFBl in the diet and liver cancer. While strong associations between AFBl exposure and liver cancer have been found, new technologies have only recently become available to monitor individual exposure and metabolism of aflatoxin Bl in people. These methods will facilitate the dissection of the role of viral agents, such as hepatitis B, from aflatoxins in the etiology of liver cancer. The development of biomarkers to assess the exposure to aflatoxin will help in efforts to limit the potential risk in human populations. In the following proposed study we will examine both seasonal and annual variabilities in aflatoxin intake, metabolism, urinary and albumin disposition in people living in the Gambia, West Africa who are at high risk to develop liver cancer. These studies will systematically probe the correspondence between dietary intake of aflatoxins with biological markers, such as excreted AFB-DNA adducts, oxidative metabolites in urine, aflatoxin Ml in human milk and covalently bound aflatoxin to serum albumin, to determine which of these markers have utility for non-invasively assessing the exposure status of people at high risk for liver cancer.