Core C

B3.分子遗传学与表观遗传学 IHSFC的分子遗传学和表观遗传学部分，以前称为设施核心C： 微阵列和分子生物学，已经转变为解决表达的当前和未来的需求， 中心成员在基因表达、表观遗传学和遗传学领域提供仪器和专业知识支持， 功能基因组学这项活动的目标是为中心成员提供接触最先进的 通过使用仪器，指导， 在数据分析方法和培训方面提供援助。我们在此基础上增加了表观遗传学分析 重复拨款，取代以前用于蛋白质组学的资源。这一决定 是因为约翰霍普金斯医学院的蛋白质组学核心是 (http：//www.hopkinsmedicine.org/msf/），我们选择不提供 重复这些努力。国际水文安全委员会这一组成部分的目标是： 提供最先进的基因表达分析，并使用Affytek平台对小鼠进行分析 （基因组430 A 2.0阵列）、大鼠（表达230 2.0）和人（基因组U133 Plus 2.0阵列）基因芯片 包括这些物种的全球基因图谱; 提供基础设施，使研究人员能够了解全球基因表达变化的修改 通过表观遗传变化表现出来的环境暴露; 与生物信息学/生物统计学核心集成，以使用生物导体和其他工具分析 遗传和表观遗传变化。

B3. Molecular Genetics and Epigenetics The Molecular Genetics and Epigenetics component of the IHSFC, formerly called Facility Core C: Microarray and Molecular Biology, has been transformed to address the expressed current and future needs of Center members for instrumentation and expertise support in the areas of gene expression, epigenetics and functional genomics. The goal of this activity is to provide to Center members access to cutting edge technologies in the analysis of gene expression and modulation through access to instrumentation, instruction, assistance in methods and training in the analysis of data. We have added the epigenetics analysis to this iteration of the grant, replacing resources that had been previously directed towards proteomics. This decision was made because the Proteomics Core at Johns Hopkins School of Medicine is (http://www.hopkinsmedicine.org/msf/) now widely available for Center members and we choose not to duplicate these efforts. The objectives of this component of the IHSFC are to: ¿ provide state-of-the-art analysis of gene expression and analysis using the Affymetrix platform for mouse (genome 430A 2.0 array), rat (expression 230 2.0) and human (genome U133 Plus 2.0 array) gene chips that comprise the global gene profiling for these respective species; ¿ provide infrastructure so that an investigator can understand the global gene expression changes modified by environmental exposures that are manifested through epigenetic changes; ¿ integrate with the Bioinformatics/Biostatistics core for using Bioconductor and other tools for the analysis of genetic and epigenetic changes.