METABOLITES IN BRAIN AND THE RELATIONSHIP TO 2-DEOXYGLUCOSE METHOD

脑内代谢物及其与2-脱氧葡萄糖法的关系

• 批准号: 4687715
• 负责人: M T HUANG
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4687715
• 关键词: anions; bioenergetics; brain metabolism; cations; chromatography; deoxyglucose; glucose; glucose 6 phosphate; glucose metabolism; hexokinase; ion exchange chromatography; lyophilization; paper chromatography; phosphorylation; radiodiagnosis; radiotracer

The purity of radioactive glucose isolated as glucose-borate complex from anion exchange column chromatography was examined in this report. The elute of the Dowex AG 1 x 8 borate column was found to be contaminated with implicity which can be removed by cation exchange chromatography. This cationic metabolite was also found to comigrate with glucose on paper chromatography. The following structural and metabolic properties were also assessed to these unidentified metabolites: that they must carry polyhydroxy group and that they are formed from glucose-6-phosphate. The time-course of the production of the anionic and cationic metabolites seem to level off at about 7 min after the injection of labeled glucose to brain through the internal carotid artery, with the radioactivity in cationic fraction at about 1/3 of that in the anionic faction. Since metabolites of glucose in glycolysis and Krebs cycle carry negative charges, the production of the cationic metabolites may represent pathways of glucose metabolism not involved ine energy production. Our results indicated that the dephosphorylation of glucose-6-phosphate may not be as large as reported previously by us. Our results also indicate that about 30% of the flux through hexokinase may be channeled through pathways not involved in energy production.

以葡萄糖-硼酸盐复合物形式从葡萄糖中分离的放射性葡萄糖的纯度 本文对阴离子交换柱色谱法进行了研究。 的 发现Dowex AG 1 x 8硼酸盐柱的柱被污染， 可通过阳离子交换色谱法去除。 这 在纸上也发现阳离子代谢产物与葡萄糖共迁移 层析 以下结构和代谢特性， 还评估了这些未鉴定的代谢物：它们必须携带 它们是由葡萄糖-6-磷酸形成的。 的 阴离子和阳离子代谢产物产生的时间过程似乎 在向脑内注射标记葡萄糖后约7 min时趋于平稳 通过颈内动脉，与放射性阳离子 部分在约1/3的阴离子部分。 由于代谢产物 糖酵解和克雷布斯循环中的葡萄糖携带负电荷， 阳离子代谢产物的产生可能代表葡萄糖 新陈代谢不涉及能量的产生。 我们的研究结果表明， 葡萄糖-6-磷酸的去磷酸化作用可能不如 我们之前报道过。 我们的研究结果还表明，大约30%的 通过己糖激酶的流量可以通过不参与 能源生产。