A Multimodal Mass Spectrometric Imaging Instrument with Enhanced Ion Characterisation Capability

这个由社区发起的挑战项目有一个明确的使命，即创建新的质谱仪器，用于对复杂样品（例如原核和真核细胞和组织）中的外源性和内源性化合物进行灵敏、快速和空间分辨的检测和结构表征。新仪器将包括具有解吸后电离的新型多模式离子源，与质量分析仪配置相结合，旨在提供最高的准确性和选择性，这是以前从未尝试或实现的。我们将构建一个独特的多模态成像质谱仪仪器，它允许生物组织的分子映射在前所未有的灵敏度，化学深度和空间分辨率。主要仪器模块包括傅立叶变换离子回旋共振光谱仪和混合捕获离子迁移率高分辨率飞行时间质量分析仪（仪器1），二次离子质谱仪与后电离（仪器2），和显微镜模式西姆斯仪器（仪器3）。为了开发实现这一目标的组件，我们设计了七个合作项目，在帝国理工学院伦敦，牛津，伯明翰和曼彻斯特的合作伙伴网站托管，并与合作伙伴机构利兹和爱丁堡以及合作伙伴布鲁克，Ionoptika，NPL和阿斯利康合作支持。

This challenge led community project has a clear mission to create new mass spectrometry instrumentation for sensitive, rapid and spatially resolved detection and structural characterisation of exogenous and endogenous compounds from complex samples (e.g. prokaryotic and eukaryotic cells and tissues). The new instruments will include novel multi-modal ion sources with post-desorption ionisation, coupled to a configuration of mass analysers, designed to deliver the highest accuracy and selectivity, which has not been attempted or achieved before. We will construct a unique multimodal imaging mass spectrometer instrument, which allows the molecular mapping of biological tissues at unprecedented sensitivity, chemical depth and spatial resolution. The main instrument modules include a Fourier-transform Ion Cyclotron Resonance spectrometer and hybrid trapped ion mobility-high-resolution time-of-flight mass analyser (instrument 1), a secondary ion mass spectrometer with post-ionisation (instrument 2), and a microscope mode SIMS instrument (instrument 3). To develop the components to deliver this we have designed seven collaborative projects, hosted at partners sites Imperial College London, Oxford, Birmingham and Manchester supported by collaborations with partner institutes Leeds and Edinburgh and collaborating partners Bruker, Ionoptika, NPL and AstraZeneca.