NMR STUDIES OF AGING IN CELLS, ORGANS, AND ANIMALS

核磁共振波谱目前正在美国国家航空航天局用于研究 人体外周肌肉磷代谢的研究。年龄- 31P核磁共振谱的相关变化和运动相关变化如下 调查。研究对象包括所有年龄段的 BLSA。 进一步的工作将集中在动物的器官生理学上。一项研究聚焦于 离体心31P代谢的研究及远期研究 生理干预及其与紧急需求和 供应压力源。另一项研究已经启动，研究对象是 孤立的灌流肺。将进行动力学过程的研究 使用磁化转移技术，这提供了进一步的洞察 磷代谢的详细调控。此外, 已经进行了方法学研究，以进一步发展 磁化传递技术本身，以及它在问题中的应用 细胞内的隔间。

NMR spectroscopy is currently being used at the NIA to study the phosphorous metabolism of peripheral muscle in human volunteers. Age- related and exercise-related changes in the 31P NMR spectrum are under investigation. Studies are performed on subjects of all ages from the BLSA. Further work will focus on organ physiology in animals. One study focuses on 31P metabolism of isolated hearts and will investigate long-term physiologic interventions and their interactions with acute demand and supply stressors. An additional study has been initiated in studies of the isolated perfused lung. Studies of kinetic processes will be performed using magnetization transfer techniques, which provide further insight into the detailed regulation of phosphorous metabolism. In addition, methodolgic studies have been undertaken to further develop the magnetization transfer technique itself, as well as to apply it to problems of intracellular compartmentation.