MOLECULAR STUDIES BY SOLID-STATE NMR

固态核磁共振光谱在非晶态的研究中是非常宝贵的 固体 我们特别感兴趣的是 基于交叉极化魔角旋转（CPMAS）的实验 法 应用集中在阐明局部构象 在一个合成的九个氨基酸序列中发现的淀粉样蛋白， 老年痴呆症 这项工作一直是一个应用旋转 共振（RR）技术，其中自旋扩散之间的广泛解决 通过输入转子能量来增强共振。 更基本的工作包括开发一个实验，其中RR 发生在定标化学位移起始处的光谱共振之间。 在 此外，我们还开发了一种基本上是TOCSY的CPMAS版本， 其中异核的化学位移被缩放到零，消除了 (in平均哈密顿理论的意义）塞曼能垒， 自旋扩散 我们称这个实验为DICSY，表示偶极相关 谱 这和缩放RR实验都是基于设计的 以及分析适当的多脉冲定标序列。

Solid-state NMR spectroscopy is invaluable in the study of non-crystalline solids. We have been particularly interested in the application of experiments based on the cross polarization-magic angle spinning (CPMAS) technique. Applications have centered on elucidation of local conformation within a synthetic nine-amino acid sequence found in the amyloid protein of Alzheimer's disease. This work has been an application of the rotational resonance (RR) technique, in which spin diffusion between widely resolved resonances is enhanced by input of rotor energy. More basic work has included development of an experiment in which RR occurs between spectral resonances at scaled chemical shift onsets. In addition, we have developed which is essentially a CPMAS version of TOCSY, in which chemical shifts of heteronuclei are scaled to zero, eliminating (in the sense of average Hamiltonian theory) the Zeeman energy barrier to spin diffusion. We call this experiment DICSY, for dipolar correlation spectroscopy. Both this and the scaled RR experiment are based on design and analysis of appropriate multiple-pulse scaling sequences.