EXPRESSION OF ROTAVIRUS OUTER CAPSID VP4 SUBUNITS (VP8 AND VP5) IN E. COLI

人VP4外衣壳蛋白的VP5和VP8裂解亚基 轮状病毒KU株(VP7 1型)、DSI株(VP72型)或无症状 人轮状病毒1076株(VP7血清2型)在大肠杆菌中表达。 各毒株VP5或VP8蛋白免疫豚鼠的研究 诱导的抗体中和VP4所来自的轮状病毒 亚基被衍生出来。交叉免疫沉淀获得的结果 用VP5和VP8抗血清进行相互中和试验 表达的蛋白质表明VP4的VP8亚基包含主要的 负责血清型特异性中和的抗原位点(S) 轮状病毒通过VP4，而VP4的VP5亚基负责 不同VP4菌株间的交叉反应观察 血清型。

The VP5 and VP8 cleavage subunits of the VP4 outer capsid protein of human rotavirus strain KU (VP7 serotype 1), DSI (VP7 serotype 2) or asymptomatic human rotavirus strain 1076 (VP7 serotype 2) were expressed in E. coli. Immunization of guinea pigs with the VP5 or VP8 protein of each strain induced antibodies that neutralized the rotavirus from which the VP4 subunits were derived. The results obtained by cross-immunoprecipitation and reciprocal neutralization assay using antisera to the VP5 and VP8 expressed proteins suggest that the VP8 subunit of VP4 contains the major antigenic site(s) responsible for serotype-specific neutralization of rotavirus via VP4, whereas the VP5 subunit of VP4 is responsible for the cross-reactivity observed among strains that belong to different VP4 serotypes.