EXPRESSION OF ROTAVIRUS OUTER CAPSID VP4 SUBUNITS (VP8 AND VP5) IN E. COLI

人VP4外衣壳蛋白的VP5和VP8切割亚基 轮状病毒株KU（VP7血清型1）、DSI（VP7血清型2）或无症状 人轮状病毒1076株（VP7血清型2）在E.杆菌 用各菌株的VP5或VP8蛋白免疫豚鼠 诱导抗体中和轮状病毒， 亚基衍生。通过交叉免疫沉淀获得的结果 和使用VP5和VP8的抗血清的相互中和测定 表达的蛋白质表明，VP4的VP8亚基含有主要的 抗原位点，负责对 轮状病毒通过VP4，而VP4的VP5亚基负责 在属于不同VP4的菌株之间观察到交叉反应性 血清型

The VP5 and VP8 cleavage subunits of the VP4 outer capsid protein of human rotavirus strain KU (VP7 serotype 1), DSI (VP7 serotype 2) or asymptomatic human rotavirus strain 1076 (VP7 serotype 2) were expressed in E. coli. Immunization of guinea pigs with the VP5 or VP8 protein of each strain induced antibodies that neutralized the rotavirus from which the VP4 subunits were derived. The results obtained by cross-immunoprecipitation and reciprocal neutralization assay using antisera to the VP5 and VP8 expressed proteins suggest that the VP8 subunit of VP4 contains the major antigenic site(s) responsible for serotype-specific neutralization of rotavirus via VP4, whereas the VP5 subunit of VP4 is responsible for the cross-reactivity observed among strains that belong to different VP4 serotypes.