VITAMIN D METABOLISM--FUNCTION OF KIDNEY DONOR/RECIPIENT

这项拟议的研究将考察老龄化对老年人的影响 肾脏恢复25(OH)D-1α-羟基酶的能力 单肾切除或肾移植后的活动。 我们实验室的初步研究表明残留物 幼年动物的肾脏(单肾切除后)表现出 显著的补偿肾质量下降的能力 肾脏25(OH)D-1α迅速和显著增加的平均值- 羟基酶活性；这种代偿反应似乎是 在老年肾脏中有限或不存在。计划的目标 实验是为了进一步表征补偿反应 单肾切除后酶活性的变化及临床意义 年龄对这一现象的影响。未来的研究将应用于 这些实验的结果对人类捐赠者和 不同年龄段的移植受者。另外，一种大鼠模型 将开发肾移植系统，以期 研究同种异体移植的年龄以及受者对 25(OH)D-1α-羟基酶活性。这样做的结果 研究将提供重要的数据，为 肾捐献者和肾移植患者治疗程序的发展 移植受者保持维生素D活性和 最大限度地减少后续的骨质流失。

The proposed research will examine the impact of aging on the ability of the kidney to recover 25(OH)D-1 alpha-hydroxylase activity following uninephrectomy or renal transplantation. Preliminary studies in our laboratory indicate that remnant kidneys (postuninephrectomy) in young animals exhibit a remarkable ability to compensate for decreased renal mass by means of a rapid and dramatic increase in renal 25(OH)D-1 alpha- hydroxylase activity; this compensatory response appears to be limited or absent in aged kidneys. The objective of the planned experiments is to further characterize the compensatory response of enzyme activity post-uninephrectomy and to explore the effects of age on this phenomenon. Future studies will apply the results of these experiments to studies of human donors and transplant recipients of various ages. In addition, a rat model system of renal transplantation will be developed in order to examine the effects of age of isograft, as well as recipient, on 25(OH)D-1 alpha-hydroxylase activity. The results of this research will provide important data essential for the development of therapeutic procedures in kidney donors and transplant recipients which preserve vitamin D activity and minimize subsequent bone loss.