VITAMIN D METABOLISM--FUNCTION OF KIDNEY DONOR/RECIPIENT

这项拟议中的研究将研究衰老对 肾脏恢复25（OH）D-1 α-羟化酶的能力 单肾切除术或肾移植后的活动。 我们实验室的初步研究表明 年轻动物的肾脏（单侧肾切除术后）表现出 通过以下方式补偿肾脏质量减少的显著能力 意味着肾脏25（OH）D-1 α- 羟化酶活性;这种代偿反应似乎是 老年肾脏有限或缺失。 计划的目标 实验是为了进一步表征补偿反应， 单肾切除术后酶活性的变化，并探讨 年龄对这种现象的影响。 今后的研究将采用 将这些实验的结果与人类供体的研究相结合， 不同年龄的移植受者。 此外， 肾移植系统将被开发， 检查同系移植物的年龄以及受体对 25（OH）D-1 α-羟化酶活性。 的结果 研究将提供重要的数据， 在肾脏捐赠者中开发治疗程序， 保持维生素D活性的移植受者， 最大限度地减少随后的骨丢失。

The proposed research will examine the impact of aging on the ability of the kidney to recover 25(OH)D-1 alpha-hydroxylase activity following uninephrectomy or renal transplantation. Preliminary studies in our laboratory indicate that remnant kidneys (postuninephrectomy) in young animals exhibit a remarkable ability to compensate for decreased renal mass by means of a rapid and dramatic increase in renal 25(OH)D-1 alpha- hydroxylase activity; this compensatory response appears to be limited or absent in aged kidneys. The objective of the planned experiments is to further characterize the compensatory response of enzyme activity post-uninephrectomy and to explore the effects of age on this phenomenon. Future studies will apply the results of these experiments to studies of human donors and transplant recipients of various ages. In addition, a rat model system of renal transplantation will be developed in order to examine the effects of age of isograft, as well as recipient, on 25(OH)D-1 alpha-hydroxylase activity. The results of this research will provide important data essential for the development of therapeutic procedures in kidney donors and transplant recipients which preserve vitamin D activity and minimize subsequent bone loss.