REDOX PROTEINS AND THEIR MODEL

氧化还原蛋白及其模型

• 批准号: 3915628
• 负责人: HIROYASU JACHIKAWA
• 金额: --
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3915628
• 关键词: DNA; Raman spectrometry; biological signal transduction; carbopolycyclic compound; charge transfer complex; chemical carcinogenesis; chemical models; cytochrome c; electrochemistry; ferredoxin; horses; nucleic acid structure; protein structure; redoxin

The main objective of our proposed project is to investigate charge transfer mechanism of proteins, nucletic acids and other model compounds. The investigators also plan to study photo- induced charge transfer processes at the interface between protein tissue and aromative hydrocarbons for the investigation of carcinogenesis. The heterogeneous charge transfer mechanism of redox proteins, such as horse heart cytochrome C (Cyt C) and ferredoxin will be examined at various electrodes. Electrochemical methods including cyclic voltammetry (CV), square wave voltammetry (SWV) and chronocoulometry Situ Raman spectroscopy will be utilized to obtain structural information of those protein molecules during the oxidation and reduction processes. Photo-induced charge transfer (or energy transfer) between proteins of tissue and aromatic hydrocarbons, such as perylene and fluoranthene will be investigated by fluorescence spectroscopy, as well a steady state fluorescence studies of the systems. This study may provide the information for the initiation reactions of carcinogenesis between aromatic mydrocarbons and protein tissue.

我们提出的项目的主要目标是调查 蛋白质、核酸等的电荷转移机理 模型化合物。 调查人员还计划研究照片- 界面处的感应电荷转移过程 蛋白质组织和芳香碳氢化合物的研究 致癌作用 氧化还原蛋白质的异质电荷转移机制， 如马心细胞色素C（Cyt C）和铁氧还蛋白， 在不同的电极上检查。 电化学方法 包括循环伏安法、方波伏安法 (SWV)和计时库仑法原位拉曼光谱将是 用于获得这些蛋白质的结构信息 分子在氧化和还原过程中。 光诱导电荷转移（或能量转移） 组织蛋白质和芳香烃，如二萘嵌苯 荧光光谱法研究荧蒽 光谱，以及稳态荧光研究的 系统. 本研究可为启动 芳烃与致癌反应 蛋白质组织