REGULATION OF GLYCOSIS IN THE AGING HEART

研究人员建议研究衰老的影响 对心脏糖酵解的控制。 具体目标 包括：1）分离、纯化和表征 6-大鼠心脏磷酸果糖2-激酶 负责合成果糖2，6-二磷酸。 2)为了确定这种酶是否来源于心脏 是双功能的，就像它在肝脏中一样， 对环腺苷酸依赖性磷酸化敏感 蛋白激酶，导致相互变化， 活动 3)研究糖酵解的整体调控 分离心脏和肌细胞的离散部分 从老年大鼠和心肌病大鼠的心脏中获得 仓鼠 这些研究将包括确定 果糖2，6-磷酸水平和活性 6-磷酸果糖2-激酶和6-磷酸果糖1-激酶。 在 同样研究糖原磷酸化酶的活性 也将被研究。 4)这些参数将 在心脏的肌细胞和成纤维细胞中测定， 为了了解果糖的水平 2，6-二磷酸和6-磷酸果糖2-激酶的活性。 由各种激素和效应物调节。 这些研究 将与项目0004中描述的那些相关联， 处理激素受体。 5)衰老老鼠的心脏 并将研究心肌病仓鼠模型， 测定心脏合成肌酸的能力 由胍基乙酸盐在胍基 甲基转移酶

The investigators propose to study the influence of aging on the control of glycolysis in heart. The specific aims include: 1) to isolate, purify and characterize the 6-phosphofructo 2-kinase of rat heart, the enzyme responsible for the synthesis of fructose 2,6-bisphosphate. 2) To determine whether the enzyme, originating from heart is bifunctional, as it is in liver, and whether the enzyme is susceptible to phosphorylation by cyclic AMP-dependent protein kinase, with resultant reciprocal changes in activities. 3) To study regulation of glycolysis in whole and separated discrete parts of the heart and im myocytes obtained from hearts of aging rats and of cardiomyopathic hamsters. These studies will include determination of levels of fructose 2,6-phosphate and the activities of 6-phosphofructo 2-kinase and 6-phosphofructo 1-kinase. In the same studies the activity of glycogen phosphorylase will also be studied. 4) These parameters will be determined in myocytes and fibroblasts of the heart, in order to learn whether the levels of fructose 2,6-bisphosphate and of 6-phosphofructose 2-kinase are modulated by various hormones and effectors. These studies will be correlated with those described in project 0004, dealing with hormone receptors. 5) Hearts from aging rats and from model cardiomyopathic hamsters will be studied to determine the capacity of the heart to synthesize creatine from guanidinoacetate under governance of guanidino methyltransferase.