REGULATION OF GLYCOSIS IN THE AGING HEART

研究人员提议研究衰老的影响 浅谈心脏糖酵解的控制。具体目标 包括：1)分离、纯化和鉴定 大鼠心脏的6-磷酸果糖2-激酶，酶 负责2，6-二磷酸果糖的合成。 2)确定酶是否来自心脏 是双功能的，就像在肝脏中一样，以及酶是否 容易被依赖于环状AMP的磷酸化 蛋白激酶，以及由此产生的相互变化 活动。3)整体研究糖酵解的规律。 分离出心脏和肌细胞的不同部分 取自衰老大鼠和心肌病大鼠的心脏 仓鼠。这些研究将包括确定 果糖2，6-磷酸水平和酶活性 6-磷酸果糖2-激酶和6-磷酸果糖1-激酶。在……里面 同样研究了糖原磷酸化酶的活性 也将进行研究。4)这些参数将是 在心脏的肌细胞和成纤维细胞中确定， 为了了解果糖水平是否 2，6-二磷酸和6-磷酸果糖2-激酶 受各种激素和效应器的调节。这些研究 将与项目0004中描述的那些相关联， 处理荷尔蒙受体。5)衰老大鼠的心脏 并将从模型心肌病仓鼠研究到 测定心脏合成肌酸的能力 从胍基乙酸胍中脱除 甲基转移酶。

The investigators propose to study the influence of aging on the control of glycolysis in heart. The specific aims include: 1) to isolate, purify and characterize the 6-phosphofructo 2-kinase of rat heart, the enzyme responsible for the synthesis of fructose 2,6-bisphosphate. 2) To determine whether the enzyme, originating from heart is bifunctional, as it is in liver, and whether the enzyme is susceptible to phosphorylation by cyclic AMP-dependent protein kinase, with resultant reciprocal changes in activities. 3) To study regulation of glycolysis in whole and separated discrete parts of the heart and im myocytes obtained from hearts of aging rats and of cardiomyopathic hamsters. These studies will include determination of levels of fructose 2,6-phosphate and the activities of 6-phosphofructo 2-kinase and 6-phosphofructo 1-kinase. In the same studies the activity of glycogen phosphorylase will also be studied. 4) These parameters will be determined in myocytes and fibroblasts of the heart, in order to learn whether the levels of fructose 2,6-bisphosphate and of 6-phosphofructose 2-kinase are modulated by various hormones and effectors. These studies will be correlated with those described in project 0004, dealing with hormone receptors. 5) Hearts from aging rats and from model cardiomyopathic hamsters will be studied to determine the capacity of the heart to synthesize creatine from guanidinoacetate under governance of guanidino methyltransferase.