PURIFY/ISOLATE/CHARACTERIZE GONADAL POLYPEPTIDES

这份合同的目标是提纯、分离和鉴定 猪卵泡液(PFF)中的黄体生成抑制物(LI)。全氟化铁 将对NIH提供的锂进行评估，以确定其锂和锂的含量 适合用作进一步提纯的起始原料。 生产部分纯化的馏分，将允许独立 生物活性的验证和附加物的评价 层析程序和适当步骤的选择(S) 这将生产出一种适合表征的“超纯”产品 学习。将生产足够的超纯产品，以允许 超纯锂的生化表征及A_2O_3的生产 可用于免疫亲和的兔多克隆抗体 提纯锂。一种足够的部分提纯材料已经被 为最终提纯而生产的超纯形式，提纯 将采用部分提纯锂的生产方案来生产 分发给科学界的材料。

The objective of this contract is to purify, isolate and characterize Luteinization Inhibitor (LI) from porcine follicular fluid (pFF). pFF provided by NIH will be evaluated to determine its content of LI and its suitability for use as the starting material for further purification. The production of a partially purified fraction, will permit independent verification of bioactivity and the evaluation of additional chromatographic procedures and the selection of the appropriate step(s) that will produce an "ultrapure" product suitable for characterization studies. Sufficient ultrapure product will be produced to permit the biochemical characterization of ultrapure LI and the production of a polyclonal antibody in rabbits suitable for use in immunoaffinity purification of LI. One sufficient partially purified material has been produced for final purification to the ultrapure form, the purification scheme for production of partially purified LI will be employed to produce material for distribution to the scientific community.