CLONING OF TUMOR CELLS ON OIL MICROCARRIER EMULSIONS

油微载体乳液上肿瘤细胞的克隆

• 批准号: 3614601
• 负责人: ROBERT W VELTRI
• 金额: $ 5万
• 依托单位: AMERICAN BIOTECHNOLOGY COMPANY, LTD
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-29 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3614601
• 关键词: biotechnology; clone cells; human tissue; neoplastic cell; tumor antigens

A major objective of this study will be to investigate the utility of new and innovative liquid microcarrier (MC) system for cloning and propagating human tumor cells in vitro. This new patented technology is an oil emulsion of defined droplet size (100-200 mm), having a smooth pliable and transparent surface. The protein at the oil emulsion interface can directly support the growth of transformed cells or these layers can be designed to contain proteins important to the attachment and spreading of normal or malignantly transformed cells. ABC, Ltd. in cooperation with G.E.'s R & D staff will prepare MC oil emulsion micro carriers conductive to the attachment and growth human tumor cells. The new MC system will be compared to the current two layer soft agar cloning methodology using primary tumor tissue samples obtained from patients at surgery. The prototype oil emulsion MC will be of value to cloning and propagating primary human tumors for use in chemosensitivity assays. In addition, this technology can provide a source of tumor cells antigens for preparing monoclonal antibodies for tumor-specific immunotherapy. Phase II will involve the application of the MC technology to the therapeutic applications stated in the areas of chemosensitivity and immunotherapy.

这项研究的一个主要目标将是调查新的 和创新的用于克隆和繁殖的液体微载体(MC)系统 体外培养的人肿瘤细胞。这项新的专利技术是一种石油 定义液滴尺寸(100-200 mm)的乳液，具有光滑、柔韧和 透明表面。油乳剂界面上的蛋白质可以 直接支持转化细胞的生长或这些层 设计成含有对依附和扩散很重要的蛋白质 正常或恶性转化的细胞。ABC股份有限公司与 通用电气S研发人员将制备MC油微载体导电 对附着和生长的人类肿瘤细胞。新的MC系统将是 与目前使用的两层软琼脂克隆方法相比 原发肿瘤组织标本取自手术患者。这个 原型油乳剂MC具有较高的克隆和繁殖价值 用于药物敏感性分析的原发人类肿瘤。此外，这一点 技术可以为制备肿瘤细胞抗原提供来源 用于肿瘤特异性免疫治疗的单抗。第二阶段将 涉及MC技术在治疗中的应用 在化疗敏感性和免疫治疗领域的应用。