CLONING OF TUMOR CELLS ON OIL MICROCARRIER EMULSIONS

本研究的一个主要目标是调查新的 和创新的液体微载体（MC）系统，用于克隆和繁殖 体外培养人肿瘤细胞。 这项新的专利技术是一种油 确定液滴尺寸（100-200 mm）的乳液，具有光滑柔韧的 透明表面 油乳液界面处的蛋白质可以 直接支持转化细胞的生长，或者这些层可以 设计成含有对附着和扩散重要的蛋白质， 正常或恶性转化的细胞。 ABC有限公司与 G.E.公司研发人员将MC油乳液微载体导电 对人类肿瘤细胞的附着和生长的影响。 新的MC系统将 与目前的双层软琼脂克隆方法相比， 手术时从患者获得的原发性肿瘤组织样品。 的 原型油乳剂MC对克隆和扩繁具有重要价值 用于化学敏感性测定的原发性人肿瘤。 另外这款 技术可以提供肿瘤细胞抗原的来源， 用于肿瘤特异性免疫治疗的单克隆抗体。 二期将 涉及MC技术在治疗中的应用， 在化学敏感性和免疫治疗领域的应用。

A major objective of this study will be to investigate the utility of new and innovative liquid microcarrier (MC) system for cloning and propagating human tumor cells in vitro. This new patented technology is an oil emulsion of defined droplet size (100-200 mm), having a smooth pliable and transparent surface. The protein at the oil emulsion interface can directly support the growth of transformed cells or these layers can be designed to contain proteins important to the attachment and spreading of normal or malignantly transformed cells. ABC, Ltd. in cooperation with G.E.'s R & D staff will prepare MC oil emulsion micro carriers conductive to the attachment and growth human tumor cells. The new MC system will be compared to the current two layer soft agar cloning methodology using primary tumor tissue samples obtained from patients at surgery. The prototype oil emulsion MC will be of value to cloning and propagating primary human tumors for use in chemosensitivity assays. In addition, this technology can provide a source of tumor cells antigens for preparing monoclonal antibodies for tumor-specific immunotherapy. Phase II will involve the application of the MC technology to the therapeutic applications stated in the areas of chemosensitivity and immunotherapy.