NUCLEAR MATRIX AND ACTIN--BIOMARKERS FOR BLADDER CANCER

核基质和肌动蛋白——膀胱癌的生物标志物

• 批准号: 3493189
• 负责人: ROBERT W VELTRI
• 金额: $ 5万
• 依托单位: UROCOR, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3493189
• 关键词: actins; antitumor antibody; biomarker; biomedical automation; bladder neoplasm; clone cells; cytodiagnosis; diagnosis design /evaluation; flow cytometry; fluorescent dye /probe; human tissue; image processing; immunofluorescence technique; microscopy; monoclonal antibody; neoplasm /cancer diagnosis; neoplastic transformation; nuclear matrix

Cytoskeletal and nuclear matrix proteins (NMPs) play an important role in the bio-chemical events involved in differentiation and transformation of mammalian cells. Certain of these markers, F/G-Actin and bladder cancer- related NMPs, provide a unique means for early identification of bladder cancer as well as assessment of field disease effects. New methods of isolating, purifying reagents and labeling these biomarkers, combined with major advances in automated image cytophotometry (both optical density and fluorescence based) provide an opportunity to develop multi-marker diagnostic assays. CytoDiagnostics Inc. (CDI), a national oncology reference laboratory, is collaborating with Matritech, Inc. to investigate the dual marker combination of Actin/NMP to identify bladder cancer cells in cytology specimens. Matritech is an expert in nuclear matrix protein chemistry, while CDI has expertise with Actin and automated image analysis. The two Companies with Oklahoma University department of urology, will investigate the utility of Actin/NMP for detection of bladder cancer, assessment of field disease dynamics, relationship to recurrence, therapeutic responsiveness, and progression. The instrumentation applied to the project will include Flow Cytometry and quantitative image cytophotometry with Zeiss-IBAS automated image system having unique software modifications.

细胞骨架蛋白和核基质蛋白（NMPs）在细胞凋亡中发挥重要作用。 分化和转化过程中的生化事件 哺乳动物细胞 某些标志物F/G肌动蛋白和膀胱癌 相关的NMPs，为膀胱癌的早期识别提供了独特的手段， 癌症以及田间疾病影响的评估。 的新方法 分离、纯化试剂并标记这些生物标志物， 自动图像细胞光度测定法的主要进展（光密度和 基于荧光）提供了开发多标记物的机会 诊断分析 CytoDiagnostics Inc. (CDI)国家肿瘤学 参考实验室正在与Matritech，Inc.合作探讨 Actin/NMP双标记物联合检测膀胱癌细胞 在细胞学标本中。 Matritech是核基质蛋白的专家 CDI拥有肌动蛋白和自动图像分析方面的专业知识。 这两家公司与俄克拉荷马州大学泌尿系，将 研究Actin/NMP用于检测膀胱癌的效用， 评估田间疾病动态，与复发的关系， 治疗反应和进展。 所用仪器 该项目将包括流式细胞术和定量图像 使用Zeiss-IBAS自动成像系统进行细胞光度测定， 软件修改。