STUDY OF MESENCHYMAL TISSUES AND THEIR DISEASES

方案1、2、3和4涉及结缔组织的机制 形态发生；(1)前胶原的包装、分泌和 组装成细胞外纤维；(2)蛋白的作用和调节 通过控制胶原酶的降解而使形态发生的降解面 蛋白多糖在发育过程中的降解与肿瘤侵袭； 多种胶原蛋白和一些非胶原蛋白大分子在组织中的定位 骨骼、心血管和眼组织的胚胎发育 通过使用各种单抗，免疫组织学和 免疫电子显微镜；(4)超分子组织的研究 通过一些生物物理和生物化学方法研究基底膜。 项目5、6、7和8概述了旨在进一步探索 复杂的细胞和体液相互作用 炎症性疾病中结缔组织的降解。项目5和 探索可溶性细胞介体的作用和细胞间的相互作用 关于释放具有降解结缔组织能力的产品 矩阵。项目7的重点是胶原蛋白的结构和组成 以及结缔组织的其他基质成分和研究因素 控制这些组织的重塑。项目8调查 调节激素的生物学效应及其作用机制 病理和非病理条件下的结缔组织。 项目9、10和11包括一项关于生物合成 利用化学合成的类异戊二烯糖制备N-糖蛋白 已知结构的磷酸盐，它们将作为受体 形成的产物的鉴定、糖肽和 疾病过程中形成的糖蛋白，以及对其作用的研究 细胞表面的唾液酸。

PROJECTS 1, 2, 3 and 4 deal with mechanisms of connective tissue morphogenesis; (1) The processes of procollagen packaging, secretion and assembly into extracellular fibrils; (2) The role and regulation of the degradative side of morphogenesis through control of collagenolysis and proteoglycan degradation in development and tumor invasion; (3) The localization of various collagen and some non collagen macromolecules in the embryonic development of the skeletal cardiovascular and ocular tissues through the use of a variety of monoclonal antibodies, immunohistologic and immunoelectron microscopy; (4) Studies on the supramolecular organization of basement membranes by a number of biophysical and biochemcal approaches. PROJECTS 5, 6, 7, and 8 outline studies designed to further explore the complex cellular and humoral interactions which are responsible for degradation of connective tissues in inflammatory diseases. Projects 5 and 6 explore the role of soluable cellular mediators and cellular interactions on release of products which have the capacity to degrade connective tissue matrices. Project 7 focuses on the structure and composition of collagens and other matrix components of connective tissue and studies factors controlling remodeling of these tissues. Project 8 investigates the biological effects and mechanisms of action of hormones which regulate connective tissues in pathological and non-pathological conditions. PROJECTS 9, 10, and 11 include a study of the biosynthesis of N-glycoproteins by use of chemically synthesized isoprenoid sugar phosphates of known structures, which will serve as acceptors for identification of the products formed, a study of glycopeptides and glycoproteins formed in diseases processes, and a study of the role of sialic acid at the surface of the cell.