STUDY OF MESENCHYMAL TISSUES AND THEIR DISEASES

项目1、2、3和4涉及结缔组织的机制 形态发生;（1）前胶原的包装，分泌和 组装成细胞外原纤维;（2）的作用和调节， 通过控制胶原蛋白溶解， 蛋白多糖降解的发展和肿瘤的侵袭;（3） 各种胶原和一些非胶原大分子在 骨骼、心血管和眼组织的胚胎发育 通过使用各种单克隆抗体，免疫组织学和 免疫电镜研究;（4）超分子结构的研究 通过一些生物物理学和生物化学方法研究基底膜。 项目5、6、7和8概述了旨在进一步探索 复杂的细胞和体液相互作用， 炎症性疾病中结缔组织的降解。 项目5和 6探索可溶性细胞介质和细胞相互作用的作用 在释放具有降解结缔组织能力的产物时 矩阵 项目7的重点是胶原蛋白的结构和组成 和结缔组织的其他基质成分， 控制这些组织的重塑 项目8调查 生物效应和激素的作用机制， 病理和非病理条件下的结缔组织。 项目9、10和11包括以下生物合成的研究： 使用化学合成的类异戊二烯糖的N-糖蛋白 已知结构的磷酸盐，其将用作 形成的产物的鉴定，糖肽的研究， 疾病过程中形成的糖蛋白，以及对糖蛋白在疾病中的作用的研究。 细胞表面的唾液酸。

PROJECTS 1, 2, 3 and 4 deal with mechanisms of connective tissue morphogenesis; (1) The processes of procollagen packaging, secretion and assembly into extracellular fibrils; (2) The role and regulation of the degradative side of morphogenesis through control of collagenolysis and proteoglycan degradation in development and tumor invasion; (3) The localization of various collagen and some non collagen macromolecules in the embryonic development of the skeletal cardiovascular and ocular tissues through the use of a variety of monoclonal antibodies, immunohistologic and immunoelectron microscopy; (4) Studies on the supramolecular organization of basement membranes by a number of biophysical and biochemcal approaches. PROJECTS 5, 6, 7, and 8 outline studies designed to further explore the complex cellular and humoral interactions which are responsible for degradation of connective tissues in inflammatory diseases. Projects 5 and 6 explore the role of soluable cellular mediators and cellular interactions on release of products which have the capacity to degrade connective tissue matrices. Project 7 focuses on the structure and composition of collagens and other matrix components of connective tissue and studies factors controlling remodeling of these tissues. Project 8 investigates the biological effects and mechanisms of action of hormones which regulate connective tissues in pathological and non-pathological conditions. PROJECTS 9, 10, and 11 include a study of the biosynthesis of N-glycoproteins by use of chemically synthesized isoprenoid sugar phosphates of known structures, which will serve as acceptors for identification of the products formed, a study of glycopeptides and glycoproteins formed in diseases processes, and a study of the role of sialic acid at the surface of the cell.