STUDY OF MESENCHYMAL TISSUES AND THEIR DISEASES

间充质组织及其疾病的研究

• 批准号: 3092314
• 负责人: JEROME GROSS
• 金额: $ 173.74万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-03-01 至 1988-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3092314
• 关键词: connective tissue development; connective tissue disorder; mesenchyme

PROJECTS 1, 2, 3 and 4 deal with mechanisms of connective tissue morphogenesis; (1) The processes of procollagen packaging, secretion and assembly into extracellular fibrils; (2) The role and regulation of the degradative side of morphogenesis through control of collagenolysis and proteoglycan degradation in development and tumor invasion; (3) The localization of various collagen and some non collagen macromolecules in the embryonic development of the skeletal cardiovascular and ocular tissues through the use of a variety of monoclonal antibodies, immunohistologic and immunoelectron microscopy; (4) Studies on the supramolecular organization of basement membranes by a number of biophysical and biochemcal approaches. PROJECTS 5, 6, 7, and 8 outline studies designed to further explore the complex cellular and humoral interactions which are responsible for degradation of connective tissues in inflammatory diseases. Projects 5 and 6 explore the role of soluable cellular mediators and cellular interactions on release of products which have the capacity to degrade connective tissue matrices. Project 7 focuses on the structure and composition of collagens and other matrix components of connective tissue and studies factors controlling remodeling of these tissues. Project 8 investigates the biological effects and mechanisms of action of hormones which regulate connective tissues in pathological and non-pathological conditions. PROJECTS 9, 10, and 11 include a study of the biosynthesis of N-glycoproteins by use of chemically synthesized isoprenoid sugar phosphates of known structures, which will serve as acceptors for identification of the products formed, a study of glycopeptides and glycoproteins formed in diseases processes, and a study of the role of sialic acid at the surface of the cell.

项目 1、2、3 和 4 涉及结缔组织的机制 形态发生； (1) 前胶原蛋白的包装、分泌和分泌过程 组装成细胞外原纤维； (2) 监管机构的作用和监管 通过控制胶原溶解和形态发生的降解方面 发育和肿瘤侵袭过程中蛋白多糖的降解； (3) 的 各种胶原蛋白和一些非胶原蛋白大分子的定位 骨骼心血管和眼组织的胚胎发育 通过使用各种单克隆抗体、免疫组织学和 免疫电子显微镜； (4)超分子组织研究 通过许多生物物理和生物化学方法来形成基底膜。 项目 5、6、7 和 8 概要研究旨在进一步探索 复杂的细胞和体液相互作用 炎症性疾病中结缔组织的退化。 项目5和 6 探索可溶性细胞介质的作用和细胞相互作用 关于具有降解结缔组织能力的产品的发布 矩阵。 项目7重点研究胶原蛋白的结构和组成 和结缔组织的其他基质成分和研究因素 控制这些组织的重塑。 项目 8 调查 调节激素的生物学效应和作用机制 病理和非病理条件下的结缔组织。 项目 9、10 和 11 包括生物合成的研究 使用化学合成的类异戊二烯糖生产 N-糖蛋白 已知结构的磷酸盐，将作为受体 形成的产物的鉴定、糖肽的研究和 疾病过程中形成的糖蛋白及其作用的研究 细胞表面的唾液酸。