THE EFFECT OF LCM VIRUS INFECTION ON INSULIN SECRETION IN PANCREAS

这一分项目建议进行以下研究，以进一步界定 持续感染在奢侈功能分化交替中的作用 细胞：(1)生长激素缺乏的定量和细胞重建 GH缺陷小鼠；(2)分析GH多肽以确定GH是否有 在病毒感染过程中结构发生改变；(3)LCMV的分析 复制；(4)测量产生的生长激素自身抗体和 其他多肽激素；(5)评估低血糖。 LCMV感染小鼠；(6)LCMV存在和分布的评价 下丘脑神经元上的抗原；以及(7)作用的评估 在持续感染的小鼠中改变体内平衡的宿主因素。 这些活体研究中的每一个都可能进一步扩展到糖尿病 糖尿病模型。计划进行的体外研究包括：(1)调查 GH-3细胞持续分泌生长激素和催乳素的变化 LCMV感染；(2)病毒对生长激素产生的嗜性评价 细胞；以及(3)生长激素基因调控的评估，即 病毒基因组(与Peter Southern博士合作)。 持续感染的细胞中胰岛素的变化和产生 LCMV与杂交瘤产生抗体的持续性变化 将接受感染LCMV的检查。

This subproject proposes the following studies to further delineate the role of persistent infections in luxury function alternation of differentiated cells: (1) Quantitation of the GH deficiency and reconstruction of GH-deficient mice; (2) Analysis of GH peptides to determine whether GH has been structurally altered during viral infection; (3) Analysis of LCMV replication; (4) Measurement of the production of autoantibodies to GH and other peptide hormones; (5) Evaluation of the hypoglycemia seen in LCMV-infected mice; (6) Evaluation of the presence and distribution of LCMV antigens on the neurons of the hypothalmus; and (7) Evaluation of the role of host factors in altering homeostasis in persistently infected mice. Each of these in vivo studies may be further extended to the diabetes mellitus model. In vitro studies planned include: (1) Investigation of alternations in production of GH and prolactin by GH-3 cells persistently infected with LCMV; (2) Evaluation of the tropism of virus for GH producing cells; and (3) Evaluation of the regulation of the GH gene, the state of the viral genome (in collaboration with Dr. Peter Southern). Alterations and production of insulin in cells persistently infected with LCMV and alterations in production of antibody by hybridomas persistently infected with LCMV will be undertaken.