NEUROANATOMY/REGENERATION RELATED TO SPINAL CORD INJURY

与脊髓损伤相关的神经解剖学/再生

• 批准号: 2662863
• 负责人: LARS OLSON
• 金额: $ 46.43万
• 依托单位: KAROLINSKA INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2662863
• 关键词: fibroblast growth factor; homologous transplantation; laboratory rat; nervous system regeneration; nervous system transplantation; neuroanatomy; neurosurgery; nonhuman therapy evaluation; spinal cord injury; therapy

In adult mammals including man, severe spinal cord injury with complete disruption of nerve fiver continuity across the lesion leads to permanent loss of function below the level of the lesion. Until recently, there had been no demonstration of structural of functional recovery across a complete spinal cord lesion in an adult mammal. Using a novel spinal cord repair strategy Drs. Cheng and Olson have demonstrated that it is possible in adult rats made paraplegic by complete spinal cord transection and removal of a 5- mm-long segment of the spinal cord at the level of To, to obtain a degree of structural and functional recovery including hindlimb locomotion, weight support and sensation. The repair strategy involves five key elements: a. The use of multiple 918) fine peripheral nerve graft bridges across the lesion: b. Rerouting regenerating nerve fibers from white matter to grey matter; using precisely defined positioning of the nerve grafts: c. Stabilizing the engrafted area with a fibrin-based tissue glue: d. The use of acidic fibroblast growth factor in slow-release form: e. External stabilization of the vertebral column. Using retrograde axon-tracing methods, it was shown that nerve fibers regenerate from many centers in the brainstem and other areas. There is also regeneration of the corticospinal tract. Based on the positive results published b Dr. Cheng and Olson (Cheng, Cao & Olson: Science 273, 510-513, 1995) new experiments are now proposed. In addition to generating a further basis for interpretation of the repair strategy, several new aspects can be studied, such as whether or not there is a altered conduction across the injured area as well as aspects of specificity and topography of function of regenerated fiber tracts.

在包括人类在内的成年哺乳动物中，严重的脊髓损伤 整个病变的神经纤维连续性完全中断， 病变水平以下的永久性功能丧失。 直到最近，还没有证据表明， 成人脊髓完全损伤后的功能恢复 哺乳动物。 使用一种新的脊髓修复策略，程博士和 奥尔森已经证明，在成年大鼠中， 截瘫通过完全脊髓横断和移除5- mm长的脊髓节段，以获得 包括后肢在内的结构和功能恢复程度 运动、重量支撑和感觉。 修复策略包括 五个关键要素： a. 使用多个精细的周围神经移植桥跨越 病变： B. 将再生神经纤维从白色物质重新布线到灰色物质 问题;使用精确定义的神经移植物定位： C. 用纤维蛋白基组织胶稳定移植区域： D. 使用酸性成纤维细胞生长因子的缓释形式： e. 脊柱的外部稳定。 利用逆行轴突追踪方法，显示神经纤维 从脑干和其他区域的许多中心再生。 皮质脊髓束也有再生。 基于Cheng博士和Olson发表的积极结果B （Cheng，Cao & Olson：Science 273，510-513，1995） 现提出。 除了为以下方面提供进一步的基础之外， 修复策略的解释，几个新的方面可以 研究，如是否有一个改变传导跨 受伤的地区以及方面的特异性和地形 再生纤维束的功能。