PNEUMOCOCCAL POLYSACCARIDE REFERENCE LABORATORY

肺炎球菌多糖参考实验室

基本信息

批准号：
2297298
负责人：
GERALD SCHIFFMAN
金额：
$ 30.3万
依托单位：
SUNY DOWNSTATE MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-06-28 至 1993-06-27
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2297298
关键词：
PNEUMOCOCCAL POLYSACCARIDE REFERENCE LABORATORY

项目摘要

The overall objective of this proposal is to maintain a Pneumococcal Polysaccharide Serological Reference Laboratory for the purpose of providing a standardized reproducible quantitative system for analyses of antibody responses to pneumococcal polysaccharide vaccines as well as determination of antibody levels during the course of natural disease, surgical intervention (such as splenectomy), drug or radiation therapy, HIV infections and other assaults on the immune system. The Pneumococcal Polysaccharide Reference Laboratory will be capable of performing radioimmunoassay analyses on sera derived from NIAID approved projects in excess of 75,000 assays per year under conditions that the results will be comparable from study to study, from year to year. The Pneumococcal Polysaccharide Reference Laboratory will have available 14C internally labelled biosynthetic pneumococcal capsular polysaccharide of each of the 23 serotypes present in the current pneumococcal vaccine. These reagents will be available to investigators at the request of the project officer. The Pneumococcal Polysaccharide Reference Laboratory will have available purified pneumococcal polysaccharides, unlabelled, available for other investigators wishing to perform ELISA assays and for use in inhibition type measurements. The Pneumococcal Polysaccharide Reference Laboratory will also have available lyophilized preparations of each of the 82 pneumococcal serotypes which can be used to prepare radioactive antigens, unlabeled capsular polysaccharides, vaccines for rabbit immunization to be used for typing or subtyping and for other studies such as opsonization, mouse protection or purification of specific antibody. The Pneumococcal Polysaccharide Reference Laboratory will have available a complete set of 48 typing sera, obtained from Staten Serum Institute, Copenhagen, Denmark, for typing organisms isolated from patients, or to check the authenticity of organisms used in collaborative studies such as opsonization, phagocytosis or mouse protection tests.

该提议的总体目的是维持肺炎球菌血清学参考实验室为了提供标准化的可重复性定量系统，用于分析抗体反应肺炎球菌多糖疫苗以及确定天然疾病过程中的抗体水平，手术干预（例如脾切除术），药物或放射治疗，HIV 感染和对免疫系统的其他攻击。这肺炎球菌多糖参考实验室将是能够对衍生的血清进行放射免疫分析来自NIAID批准的项目，每年超过75,000个测定在研究与研究的情况下相当的条件研究，每年。肺炎球菌多糖参考实验室将在内部标记14C 生物合成肺炎球菌囊囊多糖当前肺炎球菌疫苗中存在23种血清型。这些根据调查人员的要求项目官员。肺炎球菌多糖参考实验室将有可用的纯化肺炎球菌多糖未标记，可用于其他调查人员希望执行ELISA分析并用于抑制类型测量。肺炎球菌多糖参考实验室还将有可用的冻干制剂 82个肺炎球菌血清型中的每一种准备放射性抗原，未标记的囊多糖，用于打字的兔免疫疫苗或亚型和其他研究，例如调整，小鼠特定抗体的保护或纯化。这肺炎球菌多糖参考实验室将具有可提供一组48个打字血清，从史坦顿获得丹麦哥本哈根血清学院打字的生物与患者分离，或检查生物的真实性用于合作研究，例如调整，吞噬作用或鼠标保护测试。