EFFECTS OF MICROBICIDES ON CHLAMYDIAL INFECTIONS

杀菌剂对衣原体感染的影响

• 批准号: 3727814
• 负责人: MORRIS D COOPER
• 金额: --
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3727814
• 关键词: Chlamydia trachomatis; antibacterial agents; cholanate compound; detergents; disease /disorder prevention /control; epithelium; epitope mapping; guinea pigs; heparin; intermolecular interaction; mucosa; organ culture; surfactant; tissue /cell culture; topical drug application

Despite a number of stragegies for controlling the high incidence of chlamydia and the fact that an adequate treatment exists for the disease, the reported incidence approaches several million cases a year at an annual cost of approximately $2 billion a year. Control of this disease would be aided if better micobicides were identified which could be topically applied and were effective in either neutralizing the chlamydia prior to attachment or blocking cell receptors on athe surface of genital mucosal epithelium. It is the goal of this proposal to screen a number of compounds including the sulfated polysaccharides, surfactants, detergents, and bile salts for their ability to inhibit attachment and/or infectivity of Chlamydia trachomatis in a variety of model systems. One of the principal objectives of this project is to identify those antichlamydial agents which are effective in preventing infection of human cell and organ culture models. The cultured cells will include those cells routinely used for chlamydial infectivity studies (polarized an nonpolarized HeLa cell monolayers) as well as the recently described human fallopian tube organ culture of C. trachomatis infection. The specific aims are: (1) to determine the antichlamydial activity and cellular cytoxicity of a selected group of microbicidal compounds in cell and organ culture systems and (2) to determine the molecular interaction between the microbicide activity and the ligand epitope on the genital mucosal epithelium. Successful antichlamydial microbicidal agents identified in this project will also be used in project 3 for studies designed to define the determinants of chlamydial pathogenesis int he guinea pig model. The information that will be obtained in all of the projects in this proposal will aid in the identification of microbicides that are effective in protecting cells and tissues of the human female genital tract against sexually transmitted viral and bacterial pathogens.

尽管有许多条纹控制着高的发病率 衣原体以及对该疾病的适当治疗的事实， 报告的发病率每年每年接近数百万例 每年约为20亿美元。 控制这种疾病将是 如果鉴定出可以局部使用的酸脂剂，请有助于 应用并有效地在中和衣原体之前 在生殖器粘膜表面的附着或阻断细胞受体 上皮。 该提议的目的是筛选许多 包括硫化多糖，表面活性剂，洗涤剂，包括硫酸化的化合物 和胆汁盐的能力抑制依恋和/或感染力 各种模型系统中的衣原体沙眼。 中的一个 该项目的主要目标是识别那些抗聚会的目标 有效预防人类细胞和器官感染的药物 文化模型。 培养的细胞将包括通常使用的细胞 用于衣原体感染性研究（极化的非极化HELA细胞 单层）以及最近描述的人类输卵管器官 沙眼梭状芽胞庭感染的培养。 具体目的是：（1） 确定选定 细胞和器官培养系统中的一组杀生化合物，（2） 确定微生物活性和 生殖粘膜上皮上的配体表位。 成功的 在该项目中确定的抗胺型杀菌剂也将是 在项目3中用于研究，旨在定义 衣原体发病机理在豚鼠模型中。 将会 可以在本提案中的所有项目中获得 鉴定有效保护细胞和有效的微生物剂 人类女性生殖道针对性传播的组织 病毒和细菌病原体。