CORE--MACROMOLECULAR SEQUENCING AND SYNTHESIS

大分子测序和合成设施准备和 提纯脱氧核糖核苷酸和多肽。改性基层 还制备了核糖核苷酸、5‘连接脱氧核糖核苷酸等 如有要求，请提供。卡普兰综合癌症中心成员经常需要 测序引物、突变探针、插入片段、聚合酶链式反应引物等。 这些都是由这个机构准备的。此外，还有一些研究 需要针对特定蛋白质区域的抗体。此设施 制备和纯化多肽，这些多肽最终将引发 抗体反应。 大分子测序和合成设施也计划 使用应用生物系统自动测序仪进行DNA测序。 该仪器将通过自动读取DNA序列来确定DNA序列 荧光标记的(非放射性)DNA片段 在电泳胶上分离。

The Macromolecular Sequencing and Synthesis Facility prepares and purifies deoxyribonucleotides and peptides. Modified base ribonucleotides, 5'-linked deoxyribonucleotides, etc. are also prepared upon request. Kaplan Comprehensive Cancer Center members often need sequencing primers, mutant probes, insertion segments, PCR primers, etc. which are all prepared by this Facility. In addition, some studies require antibodies to specific regions of proteins. This Facility prepares and purifies the peptides which will ultimately elicit the antibody response. The Macromolecular Sequencing and Synthesis Facility also is planning to perform DNA sequencing with an Applied Biosystems automated sequencer. This instrument will determine the DNA sequence by automatically reading fluorescent labeled (non-radioactive) DNA fragments after they have been separated on an electrophoretic gel.