CORE--MACROMOLECULAR SEQUENCING AND SYNTHESIS

大分子测序和合成设施准备和 纯化脱氧核糖核苷酸和肽。修饰的碱基 还制备核糖核苷酸、5 ′-连接的脱氧核糖核苷酸等 应要求卡普兰综合癌症中心的成员经常需要 测序引物、突变探针、插入片段、PCR引物等。 这些都是由这个机构准备的。此外，一些研究 需要针对蛋白质特定区域的抗体。这个设施 制备并纯化肽， 抗体反应 高分子测序和合成设施也计划 用Applied Biosystems自动测序仪进行DNA测序。 该仪器将通过自动阅读 荧光标记的（非放射性）DNA片段， 在电泳凝胶上分离。

The Macromolecular Sequencing and Synthesis Facility prepares and purifies deoxyribonucleotides and peptides. Modified base ribonucleotides, 5'-linked deoxyribonucleotides, etc. are also prepared upon request. Kaplan Comprehensive Cancer Center members often need sequencing primers, mutant probes, insertion segments, PCR primers, etc. which are all prepared by this Facility. In addition, some studies require antibodies to specific regions of proteins. This Facility prepares and purifies the peptides which will ultimately elicit the antibody response. The Macromolecular Sequencing and Synthesis Facility also is planning to perform DNA sequencing with an Applied Biosystems automated sequencer. This instrument will determine the DNA sequence by automatically reading fluorescent labeled (non-radioactive) DNA fragments after they have been separated on an electrophoretic gel.