METHODS FOR RAPID DETECTION OF CYTOMEGALOVIRUS IN BLOOD SAMPLES

快速检测血样中巨细胞病毒的方法

• 批准号: 3752279
• 负责人: HOWARD D ENGLER
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752279
• 关键词: antiviral antibody; blood tests; communicable disease diagnosis; cytomegalovirus; diagnosis design /evaluation; human tissue; monoclonal antibody; neutrophil; opportunistic infections; polymerase chain reaction; rapid diagnosis; tissue /cell culture; virus antigen; virus protein

Cytomegalovirus (CMV) is a major cause of morbidity and mortality in the immunocompromised host, especially in those having undergone solid organ or bone marrow transplants. A simple and sensitive method for the early detection of CMV blood infection in these patients would be helpful for the medical management of the patient. Efforts will be made to determine the presence of CMV~antigen in blood by detecting the pp65 lower matrix structural protein of CMV in polymorphonuclear leukocytes. Detection of the antigen with monoclonal antibodies may be useful as an early predictor of CMV infection. Comparisons of the sensitivity, specificity, speed, and ease of determining CMV antigenemia will be made with the shell vial viral culture and with a polymerase chain reaction assay utilizing the immediate early gene region of CMV for primer recognition.

巨细胞病毒（CMV）是一个主要的原因，发病率和死亡率， 免疫功能低下的宿主，特别是那些经历过实体瘤的宿主， 器官或骨髓移植一种简便、灵敏的测定 这些患者中CMV血液感染的早期检测将 有助于患者的医疗管理。着力 通过检测血液中CMV抗原的存在， pp65降低CMV基质结构蛋白多态性 白细胞用单克隆抗体检测抗原可以是 可作为CMV感染的早期预测指标。的比较 灵敏度、特异性、速度和易于确定CMV 将用壳瓶病毒培养物和 利用立即早期基因的聚合酶链反应测定 CMV区域用于引物识别。