METHODS FOR RAPID DETECTION OF CYTOMEGALOVIRUS IN BLOOD SAMPLES

巨细胞病毒(CMV)是中国人发病和死亡的主要原因。 免疫受损的宿主，尤其是在那些经历了 器官或骨髓移植。一种简便、灵敏的检测方法 在这些患者中及早发现CMV血液感染将是 有助于患者的医疗管理。我们将努力 通过检测来确定血液中是否存在巨细胞病毒抗原 CMV中性粒细胞中pp65下基质结构蛋白 白细胞。用单抗检测抗原可能是 可作为CMV感染的早期预测指标。两个版本之间的比较 检测CMV的敏感性、特异性、快速性和简便性 抗原血症将用贝壳小瓶病毒培养和 利用即刻早期基因的聚合酶链式反应 用于引物识别的CMV区域。

Cytomegalovirus (CMV) is a major cause of morbidity and mortality in the immunocompromised host, especially in those having undergone solid organ or bone marrow transplants. A simple and sensitive method for the early detection of CMV blood infection in these patients would be helpful for the medical management of the patient. Efforts will be made to determine the presence of CMV~antigen in blood by detecting the pp65 lower matrix structural protein of CMV in polymorphonuclear leukocytes. Detection of the antigen with monoclonal antibodies may be useful as an early predictor of CMV infection. Comparisons of the sensitivity, specificity, speed, and ease of determining CMV antigenemia will be made with the shell vial viral culture and with a polymerase chain reaction assay utilizing the immediate early gene region of CMV for primer recognition.